Genetic background of attenuated Salmonella typhimurium has profound influence on infection and cytokine patterns in human dendritic cells.

Details

Serval ID
serval:BIB_AA4F02331207
Type
Article: article from journal or magazin.
Collection
Publications
Title
Genetic background of attenuated Salmonella typhimurium has profound influence on infection and cytokine patterns in human dendritic cells.
Journal
Journal of Leukocyte Biology
Author(s)
Dreher D., Kok M., Cochand L., Kiama S.G., Gehr P., Pechère J.C., Nicod L.P.
ISSN
0741-5400[print], 0741-5400[linking]
Publication state
Published
Issued date
2001
Volume
69
Number
4
Pages
583-589
Language
english
Abstract
Salmonella typhimurium (ST) can cause infection in man, and attenuated strains are under consideration as live vaccine vectors. However, little is known about the interaction of ST with human dendritic cells (DC). Here, we compared the consequences of exposure of human, monocyte-derived DC with different attenuated strains of ST. Infection was observed with all four strains tested (wild type, PhoP-, PhoPc, and AroA), but the PhoPc strain was by far the most efficient. Intracellular persistence of wild type and PhoP- was longer than that of PhoPc and AroA, both of which were largely eliminated within 24 h. Most DC survived infection by the attenuated strains, although apoptosis was observed in a fraction of the exposed cells. All strains induced DC maturation, independent from the extent of infection. Although all strains stimulated secretion of TNF-alpha and IL-12 strongly, PhoPc induced significantly less IL-10 than the other three strains and as much as 10 times less IL-10 than heat-killed PhoPc, suggesting that this mutant suppressed the secretion of IL-10 by the DC. These data indicate that infectivity, bacterial elimination, and cytokine secretion in human DC are controlled by the genetic background of ST.
Keywords
3-Phosphoshikimate 1-Carboxyvinyltransferase, Alkyl and Aryl Transferases/deficiency, Alkyl and Aryl Transferases/genetics, Apoptosis, Bacterial Proteins/genetics, Bacterial Proteins/physiology, Cell Differentiation, Cells, Cultured, Cytokines/secretion, Dendritic Cells/microbiology, Dendritic Cells/secretion, Genes, Bacterial, Humans, Interleukins/secretion, Monocytes/cytology, Necrosis, Salmonella typhimurium/genetics, Salmonella typhimurium/pathogenicity, Tumor Necrosis Factor-alpha/secretion, Virulence/genetics
Pubmed
Web of science
Create date
19/02/2010 18:59
Last modification date
20/08/2019 15:14
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