Chromatographic method for diaminopimelic acid detection in calcareous rocks. Presence of a bacterial biomarker in stromatolites

Details

Serval ID
serval:BIB_AA236A42A361
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Chromatographic method for diaminopimelic acid detection in calcareous rocks. Presence of a bacterial biomarker in stromatolites
Journal
J Chromatogr A
Author(s)
Borruat  G., Roten  C. A., Marchant  R., Fay  L. B., Karamata  D.
ISSN
0021-9673 (Print)
Publication state
Published
Issued date
07/2001
Volume
922
Number
1-2
Pages
219-24
Notes
Journal Article --- Old month value: Jul 13
Abstract
The presence in the environment of diaminopimelic acid (DAP), a specific eubacterial marker, can be attributed to that of bacteria. We report a reliable and highly sensitive method for the quantification of DAP in calcareous rocks. It consists of acid hydrolysis of rock powder, purification of DAP by chromatography on Dowex 50W and Spherogel AA-NA+ columns, and quantitative analysis by high-performance liquid chromatography. Addition of tritiated DAP, the internal standard, allows one to follow the relevant fractions throughout the purification procedure and to determine their yield. The analytical step consists in pre-column derivatization with ortho-phthaldialdehyde of purified samples, and separation through a reversed-phase C18 column. Chemical controls, i.e., oxidation of samples to rule out the presence of co-eluting lanthionine and cystathionine, as well as mass spectrometry, confirm the presence of DAP in analyzed samples. Our method allows the separation of meso- from L- and/or D-stereoisomers of DAP, and reveals their presence in the examined rocks, two stromatolites of different age and geographic origin.
Keywords
*Biological Markers Chromatography, High Pressure Liquid/*methods Diaminopimelic Acid/*analysis Escherichia coli/chemistry *Geology Mass Spectrometry
Pubmed
Web of science
Create date
24/01/2008 15:07
Last modification date
20/08/2019 16:14
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