Loss of glucose-induced insulin secretion and GLUT2 expression in transplanted beta-cells.

Details

Serval ID
serval:BIB_AA1E40B1967F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Loss of glucose-induced insulin secretion and GLUT2 expression in transplanted beta-cells.
Journal
Diabetes
Author(s)
Ogawa Y., Noma Y., Davalli A.M., Wu Y.J., Thorens B., Bonner-Weir S., Weir G.C.
ISSN
0012-1797[print], 0012-1797[linking]
Publication state
Published
Issued date
01/1995
Volume
44
Number
1
Pages
75-79
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Either 200 or 400 syngeneic islets were transplanted under the kidney capsule of normal or streptozocin-induced diabetic B6/AF1 mice. The diabetic mice with 400 islets became normoglycemic, but those with 200 islets, an insufficient number, were still diabetic after the transplantation (Tx). Two weeks after Tx, GLUT2 expression in the islet grafts was evaluated by immunofluorescence and Western blots, and graft function was examined by perfusion of the graft-bearing kidney. Immunofluorescence for GLUT2 was dramatically reduced in the beta-cells of grafts with 200 islets exposed to hyperglycemia. However, it was plentiful in grafts with 400 islets in a normoglycemic environment. Densitometric analysis of Western blots on graft homogenates demonstrated that GLUT2 protein levels in the islets, when exposed to chronic hyperglycemia for 2 weeks, were decreased to 16% of those of normal recipients. Moreover, these grafts had defective glucose-induced insulin secretion, while the effects of arginine were preserved. We conclude that GLUT2 expression in normal beta-cells is promptly down-regulated during exposure to hyperglycemia and may contribute to the loss of glucose-induced secretion of diabetes.
Keywords
Animals, Blotting, Western, Densitometry, Fluorescent Antibody Technique, Glucose/pharmacology, Glucose Transporter Type 2, Hyperglycemia/metabolism, Hyperglycemia/physiopathology, Insulin/secretion, Islets of Langerhans/chemistry, Islets of Langerhans/metabolism, Islets of Langerhans Transplantation/pathology, Male, Mice, Monosaccharide Transport Proteins/analysis, Monosaccharide Transport Proteins/metabolism
Pubmed
Web of science
Create date
24/01/2008 13:41
Last modification date
20/08/2019 15:14
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