Chronic bronchial allergic inflammation increases alveolar liquid clearance by TNF-alpha -dependent mechanism

Details

Serval ID
serval:BIB_AA1344928BF7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Chronic bronchial allergic inflammation increases alveolar liquid clearance by TNF-alpha -dependent mechanism
Journal
Am J Physiol Lung Cell Mol Physiol
Author(s)
Tillie-Leblond I., Guery B. P., Janin A., Leberre R., Just N., Pittet J. F., Tonnel A. B., Gosset P.
ISSN
1040-0605 (Print)
ISSN-L
1040-0605
Publication state
Published
Issued date
12/2002
Volume
283
Number
6
Pages
L1303-9
Language
english
Notes
Tillie-Leblond, Isabelle
Guery, Benoit P H
Janin, Anne
Leberre, Rozenn
Just, Nicolas
Pittet, Jean-Francois
Tonnel, Andre-Bernard
Gosset, Philippe
eng
Am J Physiol Lung Cell Mol Physiol. 2002 Dec;283(6):L1303-9. doi: 10.1152/ajplung.00147.2002. Epub 2002 Aug 9.
Abstract
Bronchial inflammation in allergic asthma is associated with active exudation from the bronchial tree into the interstitial space of both mucosa and submucosa. The aim of this study was to evaluate epithelial and endothelial permeability as well as alveolar fluid movement in a model of chronic allergic inflammation in Brown-Norway rats sensitized and challenged with ovalbumin (OA). Control groups were challenged with saline solution (C), and rats were immunized by OA but not challenged (Se). Lung sections showed a marked inflammatory infiltrate associated with perivascular and peribronchiolar edema in OA. To measure alveolar liquid clearance, a 5% bovine albumin solution with 1 microCi of (125)I-labeled human albumin was instilled into the air spaces. Alveolar-capillary barrier permeability was evaluated by intravascular injection of 1 microCi of (131)I-labeled albumin. Endothelial permeability was significantly increased in OA, from 0.08 +/- 0.01 in the C group to 0.19 +/- 0.03 in OA group (P < 0.05). Final-to-initial protein ratio was also statistically higher in OA (1.6 +/- 0.05) compared with C (1.38 +/- 0.03, P = 0.01) and Se groups (1.42 +/- 0.03, P = 0.04). Administration of anti-tumor necrosis factor-alpha antibodies within the instillate significantly decreased this ratio (1.32 +/- 0.08, P = 0.003 vs. OA). To conclude, we demonstrated a tumor necrosis factor-alpha-dependent increase in alveolar fluid movement in a model of severe bronchial allergic inflammation associated with endothelial and epithelial leakage.
Keywords
Albumins/pharmacokinetics, Animals, Blood-Air Barrier, Body Fluids/metabolism, Bronchitis/*immunology/*metabolism/pathology, Capillaries/metabolism, Capillary Permeability, Cattle, Chronic Disease, Humans, Hypersensitivity/*complications, Immunization, Immunoglobulin E/metabolism, Lung/metabolism, Male, Ovalbumin/immunology, Pulmonary Alveoli/blood supply/*metabolism, Rats, Rats, Inbred BN, Tumor Necrosis Factor-alpha/*physiology
Pubmed
Create date
29/04/2021 9:59
Last modification date
30/04/2021 5:38
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