Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.

Details

Ressource 1Download: Genes Dev.-2018-Dergai-711-22.pdf (3049.19 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_A9F33414833D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.
Journal
Genes & development
Author(s)
Dergai O., Cousin P., Gouge J., Satia K., Praz V., Kuhlman T., Lhôte P., Vannini A., Hernandez N.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Publication state
Published
Issued date
01/05/2018
Peer-reviewed
Oui
Volume
32
Number
9-10
Pages
711-722
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.
Keywords
HEK293 Cells, Humans, Mutation, Promoter Regions, Genetic, Protein Binding, Protein Domains, Protein Transport, RNA Polymerase II/metabolism, RNA Polymerase III/metabolism, RNA, Small Nuclear/genetics, RNA, Small Nuclear/metabolism, TATA Box/genetics, TATA-Box Binding Protein/metabolism, Transcription Factor TFIIB/metabolism, Transcription Factors/metabolism, BRF2, SNAPc, TBP, TFIIA, TFIIB, small nuclear RNA promoters
Pubmed
Web of science
Open Access
Yes
Create date
24/05/2018 17:46
Last modification date
20/08/2019 16:14
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