Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS.

Details

Serval ID
serval:BIB_A9702E4751B4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS.
Journal
Journal of Pharmaceutical and Biomedical Analysis
Author(s)
Choong E., Rudaz S., Kottelat A., Guillarme D., Veuthey J.L., Eap C.B.
ISSN
1873-264X[electronic]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
50
Number
5
Pages
1000-1008
Language
english
Abstract
A simple and sensitive LC-MS method was developed and validated for the simultaneous quantification of aripiprazole (ARI), atomoxetine (ATO), duloxetine (DUL), clozapine (CLO), olanzapine (OLA), sertindole (STN), venlafaxine (VEN) and their active metabolites dehydroaripiprazole (DARI), norclozapine (NCLO), dehydrosertindole (DSTN) and O-desmethylvenlafaxine (OVEN) in human plasma. The above mentioned compounds and the internal standard (remoxipride) were extracted from 0.5 mL plasma by solid-phase extraction (mix mode support). The analytical separation was carried out on a reverse phase liquid chromatography at basic pH (pH 8.1) in gradient mode. All analytes were monitored by MS detection in the single ion monitoring mode and the method was validated covering the corresponding therapeutic range: 2-200 ng/mL for DUL, OLA, and STN, 4-200 ng/mL for DSTN, 5-1000 ng/mL for ARI, DARI and finally 2-1000 ng/mL for ATO, CLO, NCLO, VEN, OVEN. For all investigated compounds, good performance in terms of recoveries, selectivity, stability, repeatability, intermediate precision, trueness and accuracy, was obtained. Real patient plasma samples were then successfully analysed.
Keywords
Method development, Quantification, Atypical antipsychotics, Antidepressants, SPE, LC-MS, Validation, Tandem Mass-Spectrometry, Solid-Phase Extraction, Liquid-Chromatographic Method, Bioanalytical Methods Validation, Pressure Chemical-Ionization, Ligand-Binding Assays, Lc-Esi-Ms, Quantitative-Determination, Atypical Antipsychotics, Ultraviolet Detection
Pubmed
Web of science
Create date
01/10/2009 16:52
Last modification date
20/08/2019 16:13
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