Liquid chromatography-high resolution mass spectrometry for broad-spectrum drug screening of dried blood spot as microsampling procedure.

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State: Serval
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_A93B0FBC6297
Type
Article: article from journal or magazin.
Collection
Publications
Title
Liquid chromatography-high resolution mass spectrometry for broad-spectrum drug screening of dried blood spot as microsampling procedure.
Journal
Analytica chimica acta
Author(s)
Joye T., Sidibé J., Déglon J., Karmime A., Sporkert F., Widmer C., Favrat B., Lescuyer P., Augsburger M., Thomas A.
ISSN
1873-4324 (Electronic)
ISSN-L
0003-2670
Publication state
Published
Issued date
31/07/2019
Peer-reviewed
Oui
Volume
1063
Pages
110-116
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Hyphenation of liquid chromatography (LC) with high-resolution mass spectrometry (HRMS) offers the potential to develop broad-spectrum screening procedures from low volumes of biological matrices. In parallel, dried blood spot (DBS) has become a valuable tool in the bioanalysis landscape to overcome conventional blood collection issues. Herein, we demonstrated the applicability of DBS as micro-sampling procedure for broad-spectrum toxicological screening.
A method was developed on a HRMS system in data dependant acquisition (DDA) mode using an extensive inclusion list to promote collection of relevant data. 104 real toxicology cases were analysed, and the results were cross-validated with one published and one commercial screening procedures. Quantitative MRM analyses were also performed on identified substances on a triple quadrupole instrument as a complementary confirmation procedure.
The method showed limits of identification (LOIs) in appropriateness with therapeutic ranges for all the classes of interest. Applying the three screening approaches on 104 real cases, 271 identifications were performed including 14 and 6 classes of prescribed and illicit drugs, respectively. Among the detected substances, 23% were only detected by the proposed method. Based on confirmatory analyses, we demonstrated that the use of blood micro-samples did not impair the sensitivity allowing more identifications in the low concentration ranges.
A LC-HRMS assay was successfully developed for toxicological screening of blood microsamples demonstrating a high identification power at low concentration ranges. The validation procedure and the analysis of real cases demonstrated the potential of this assay by supplementing screening approaches of reference.
Keywords
Analytical Chemistry, Spectroscopy, Biochemistry, Environmental Chemistry, Data dependent acquisition, Dried blood spot, General unknown screening, High resolution mass spectrometry, Microsampling
Pubmed
Web of science
Create date
27/02/2019 11:30
Last modification date
30/04/2019 7:35
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