Visualization of RNA polymerase II ternary transcription complexes formed in vitro on a Xenopus laevis vitellogenin gene.

Details

Serval ID
serval:BIB_A7761DB8D1C9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Visualization of RNA polymerase II ternary transcription complexes formed in vitro on a Xenopus laevis vitellogenin gene.
Journal
EMBO Journal
Author(s)
ten Heggeler B., Wahli W.
ISSN
0261-4189[print], 0261-4189[linking]
Publication state
Published
Issued date
09/1985
Volume
4
Number
9
Pages
2269-2273
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Stable ternary transcription complexes assembled in vitro, using a HeLa whole-cell extract, have been isolated and visualized by electron microscopy. The formation of these stable complexes on the DNA fragment used as template, the 5' end region of the Xenopus laevis vitellogenin gene B2, depends on factors present in the whole-cell extract, RNA polymerase II and at least two nucleotides. Interestingly, bending in the DNA fragment was frequently observed at the binding site of RNA polymerase II. Dinucleotides that can prime initiation within a short sequence of approximately 10 contiguous nucleotides centered around the initiation site used in vivo, also favour the formation of stable complexes. In addition, pre-initiation complexes were isolated and it was shown that factors in the extract involved in their formation are more abundant than the RNA polymerase II molecules available for binding. The possible implication of this observation relative to the in vivo situation is discussed.
Keywords
Animals, Cattle, DNA Restriction Enzymes, Genes, Hela Cells/metabolism, Humans, Kinetics, Macromolecular Substances, Microscopy, Electron, RNA Polymerase II/metabolism, Thymus Gland/enzymology, Transcription, Genetic, Vitellogenins/genetics, Xenopus
Pubmed
Web of science
Create date
24/01/2008 16:04
Last modification date
20/08/2019 15:12
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