Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays.

Details

Serval ID
serval:BIB_A6147D10F309
Type
Article: article from journal or magazin.
Collection
Publications
Title
Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays.
Journal
Journal of Pathology
Author(s)
Ullmann R., Morbini P., Halbwedl I., Bongiovanni M., Gogg-Kammerer M., Papotti M., Gabor S., Renner H., Popper H.H.
ISSN
0022-3417 (Print)
ISSN-L
0022-3417
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
203
Number
3
Pages
798-807
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
With the appearance of defect-targeted therapies, the definition of tumour protein expression profiles has gained increasing importance. Two lung carcinoma tissue microarrays, one including 75 primary adenocarcinomas (ACs) and the other comprising 67 primary squamous cell carcinomas (SQCCs), were generated in the present study. On both arrays, each tumour was represented by an average of five cores. In addition, one punch of normal lung parenchyma adjacent to each tumour was included in the array. Immunohistochemical expression of 86 proteins was evaluated and the results were analysed by non-parametric tests, hierarchical clustering, and principal component analysis. In both tumour entities, parenchyma and tumours were clearly separated by hierarchical clustering. By the same statistical approach, it was possible to distinguish ACs from SQCCs with 98% accuracy and to distinguish parenchyma adjacent to ACs from that adjacent to SQCCs with 96% accuracy. It was also possible to separate ACs into three groups that significantly differed in survival. Cathepsin E and hsp105 were identified as previously unknown predictors of survival in lung AC. In summary, this study has shown that protein profiles are feasible tools for anticipating biological behaviour.
Keywords
Adenocarcinoma/metabolism, Adenocarcinoma/pathology, Adult, Aged, Carcinoma, Squamous Cell/metabolism, Carcinoma, Squamous Cell/pathology, Cathepsin E/metabolism, Female, Gene Expression Profiling, HSP110 Heat-Shock Proteins, HSP70 Heat-Shock Proteins/metabolism, Humans, Lung Neoplasms/genetics, Lung Neoplasms/metabolism, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Proteins/genetics, Neoplasm Proteins/metabolism, Principal Component Analysis, Protein Array Analysis, Tumor Markers, Biological/genetics, Tumor Markers, Biological/metabolism
Pubmed
Web of science
Create date
05/02/2015 14:30
Last modification date
20/08/2019 15:11
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