Gas-filled microbubble-mediated delivery of antigen and the induction of immune responses.

Details

Serval ID
serval:BIB_A5FEA16169DF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Gas-filled microbubble-mediated delivery of antigen and the induction of immune responses.
Journal
Biomaterials
Author(s)
Bioley G., Lassus A., Bussat P., Terrettaz J., Tranquart F., Corthésy B.
ISSN
1878-5905 (Electronic)
ISSN-L
0142-9612
Publication state
Published
Issued date
2012
Volume
33
Number
25
Pages
5935-5946
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
The use of well characterized recombinant or purified protein antigens (Ag) for vaccination is of interest for safety reasons and in the case where inactivated pathogens are not available (cancer, allergy). However it requires the addition of adjuvants such as Ag carrier or immune stimulators to potentiate their immunogenicity. In this study, we demonstrated that gas-filled microbubbles (MB) can serve as an efficient Ag delivery system to promote phagocytosis of the model Ag ovalbumin (OVA) without the need of ultrasound application. Once internalized by DC, OVA was processed and presented to both CD4 and CD8 T cells in vitro; such observations were coupled with the capacity of MB to activate DC. In vivo administration of MB-associated OVA in naïve wild-type Balb/c mice resulted in the induction of OVA-specific antibody and T cell responses. Detailed characterization of the generated immune response demonstrated the production of both IgG1 and IgG2a serum antibodies, as well as the secretion of IFN-γ and IL-10 by splenocytes. Interestingly, similar results were obtained with human DC in regards of Ag delivery and cell activation. Therefore, the data presented here settle the proof of principle for the further evaluation of MB-based immunomodulation studies.
Pubmed
Web of science
Create date
27/08/2012 17:10
Last modification date
20/08/2019 15:11
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