Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis

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Ressource 1Download: serval:BIB_A54D80E84479.P001 (1899.09 [Ko])
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Serval ID
serval:BIB_A54D80E84479
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis
Journal
Journal of Infectious Diseases
Author(s)
Corthesy  B., Boris  S., Isler  P., Grangette  C., Mercenier  A.
ISSN
0022-1899 (Print)
Publication state
Published
Issued date
10/2005
Volume
192
Number
8
Pages
1441-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
BACKGROUND: The development of an efficacious vaccine against infection with Helicobacter pylori, the causative agent of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma, remains a challenge. Since the use of mucosal adjuvants is limited in human application, we have evaluated the potential of recombinant Lactobacillus strains producing H. pylori urease B (UreB) subunit to deliver this antigen to the gastrointestinal tract. METHODS: Mice were injected orally 3 times with a triple dose of recombinant Lactobacillus plantarum NCIMB8826, the recombinant isogenic cell-wall mutant (alr(-) MD007 strain) expressing UreB, or a mixture of recombinant UreB and cholera toxin (rUreB/CT) as a control. Urease-specific seric immunoglobulin (Ig) G and IgA were measured by use of an enzyme-linked immunosorbent assay. After challenge with Helicobacter felis, stomach infection was examined by use of the rapid urease test and by polymerase chain reaction detection of Helicobacter genomic DNA. RESULTS: Intragastric immunization with both recombinant Lactobacillus strains and rUreB/CT elicited UreB-specific antibodies. After challenge, reduction of H. felis load in the stomachs of mice was observed only after immunization with the recombinant mutant strain MD007 or with rUreB/CT. CONCLUSIONS: This is the first report of successful induction of partial protection against H. felis with a mucosal prime-boost regimen in which recombinant Lactobacillus strains were used as antigen-delivery vehicles.
Keywords
Adjuvants, Immunologic Administration, Oral Animals Antibody Formation Bacterial Vaccines/*administration & dosage/immunology Disease Models, Animal Helicobacter/isolation & purification Helicobacter Infections/immunology/*prevention & control Helicobacter pylori/*enzymology/genetics Immunoglobulin A, Secretory/*biosynthesis/blood Immunoglobulin G/biosynthesis/blood Lactobacillus/genetics/*immunology Mice Recombinant Proteins/administration & dosage/immunology Urease/*administration & dosage/biosynthesis/genetics/immunology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:53
Last modification date
25/09/2019 7:10
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