Potentiation of polarized intestinal Caco-2 cell responsiveness to probiotics complexed with secretory IgA.

Details

Serval ID
serval:BIB_A4AEE878D51A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Potentiation of polarized intestinal Caco-2 cell responsiveness to probiotics complexed with secretory IgA.
Journal
Journal of Biological Chemistry
Author(s)
Mathias A., Duc M., Favre L., Benyacoub J., Blum S., Corthésy B.
ISSN
1083-351X[electronic], 0021-9258[linking]
Publication state
Published
Issued date
2010
Volume
285
Number
44
Pages
33906-33913
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
The precise mechanisms underlying the interaction between intestinal bacteria and the host epithelium lead to multiple consequences that remain poorly understood at the molecular level. Deciphering such events can provide valuable information as to the mode of action of commensal and probiotic microorganisms in the gastrointestinal environment. Potential roles of such microorganisms along the privileged target represented by the mucosal immune system include maturation prior, during and after weaning, and the reduction of inflammatory reactions in pathogenic conditions. Using human intestinal epithelial Caco-2 cell grown as polarized monolayers, we found that association of a Lactobacillus or a Bifidobacterium with nonspecific secretory IgA (SIgA) enhanced probiotic adhesion by a factor of 3.4-fold or more. Bacteria alone or in complex with SIgA reinforced transepithelial electrical resistance, a phenomenon coupled with increased phosphorylation of tight junction proteins zonula occludens-1 and occludin. In contrast, association with SIgA resulted in both enhanced level of nuclear translocation of NF-κB and production of epithelial polymeric Ig receptor as compared with bacteria alone. Moreover, thymic stromal lymphopoietin production was increased upon exposure to bacteria and further enhanced with SIgA-based complexes, whereas the level of pro-inflammatory epithelial cell mediators remained unaffected. Interestingly, SIgA-mediated potentiation of the Caco-2 cell responsiveness to the two probiotics tested involved Fab-independent interaction with the bacteria. These findings add to the multiple functions of SIgA and underscore a novel role of the antibody in interaction with intestinal bacteria.
Keywords
Bacterial Adhesion, Bifidobacterium/metabolism, Caco-2 Cells, Epithelial Cells/cytology, Humans, Immunoglobulin A, Secretory/chemistry, Intestines/cytology, Lactobacillus/metabolism, Membrane Proteins/chemistry, Membrane Proteins/metabolism, Models, Biological, NF-kappa B/metabolism, Phosphoproteins/metabolism, Phosphorylation, Probiotics/chemistry, Tight Junctions
Pubmed
Web of science
Open Access
Yes
Create date
22/12/2010 9:59
Last modification date
20/08/2019 16:10
Usage data