Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips.

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Ressource 1Download: Roggo_et_al-2018-Environmental_Microbiology.pdf (1620.11 [Ko])
State: Public
Version: Final published version
Secondary document(s)
Download: Roggo_SI.pdf (5264.19 [Ko])
State: Public
Version: Supplementary document
Serval ID
serval:BIB_A44B85643995
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips.
Journal
Environmental microbiology
Author(s)
Roggo C., Picioreanu C., Richard X., Mazza C., van Lintel H., van der Meer J.R.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Publication state
Published
Issued date
01/2018
Peer-reviewed
Oui
Volume
20
Number
1
Pages
241-258
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Whole-cell bacterial bioreporters are proposed as alternatives to chemical analysis of, for example, pollutants in environmental compartments. Commonly based on reporter gene induction, bioreporters produce a detectable signal within 30 min to a few hours after exposure to the chemical target, which is impractical for applications aiming at a fast response. In an attempt to attain faster readout but maintain flexibility of chemical targeting, we explored the concept for quantitative chemical sensing by bacterial chemotaxis. Chemotaxis was quantified from enrichment of cells across a 600 µm-wide chemical gradient stabilized by parallel flow in a microfluidic chip, further supported by transport and chemotaxis steady state and kinetic modelling. As proof-of-concept, we quantified Escherichia coli chemotaxis towards serine, aspartate and methylaspartate as a function of attractant concentration and exposure time. E. coli chemotaxis enrichment increased sharply between 0 and 10 µM serine, before saturating at 100 µM. The chemotaxis accumulation rate was maximal at 10 µM serine, leading to observable cell enrichment within 5 min. The potential application for biosensing of environmental toxicants was investigated by quantifying chemotaxis of Cupriavidus pinatubonensis JMP134 towards the herbicide 2,4-dichlorophenoxyacetate. Our results show that bacterial chemotaxis can be quantified on a scale of minutes and may be used for developing faster bioreporter assays.
Keywords
2,4-Dichlorophenoxyacetic Acid/analysis, Aspartic Acid/analysis, Biosensing Techniques/methods, Chemotaxis/physiology, Cupriavidus/physiology, Environmental Pollutants/analysis, Escherichia coli/physiology, Herbicides/analysis, Microfluidics/methods, Serine/chemistry
Pubmed
Web of science
Open Access
Yes
Create date
22/11/2017 10:24
Last modification date
20/08/2019 16:09
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