Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins.

Details

Serval ID
serval:BIB_A447FF60F5B5
Type
Article: article from journal or magazin.
Collection
Publications
Title
Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins.
Journal
Journal of Vascular Surgery
Author(s)
Berard X., Déglise S., Alonso F., Saucy F., Meda P., Bordenave L., Corpataux J.M., Haefliger J.A.
ISSN
1097-6809 (Electronic)
ISSN-L
0741-5214
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
57
Number
5
Pages
1371-1382
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
BACKGROUND: Human saphenous vein grafts are one of the salvage bypass conduits when endovascular procedures are not feasible or fail. Understanding the remodeling process that venous grafts undergo during exposure to arterial conditions is crucial to improve their patency, which is often compromised by intimal hyperplasia. The precise role of hemodynamic forces such as shear stress and arterial pressure in this remodeling is not fully characterized. The aim of this study was to determine the involvement of arterial shear stress and pressure on vein wall remodeling and to unravel the underlying molecular mechanisms.
METHODS: An ex vivo vein support system was modified for chronic (up to 1 week), pulsatile perfusion of human saphenous veins under controlled conditions that permitted the separate control of arterial shear stress and different arterial pressure (7 mm Hg or 70 mm Hg).
RESULTS: Veins perfused for 7 days under high pressure (70 mm Hg) underwent significant development of a neointima compared with veins exposed to low pressure (7 mm Hg). These structural changes were associated with altered expression of several molecular markers. Exposure to an arterial shear stress under low pressure increased the expression of matrix metalloproteinase (MMP)-2 and MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1 at the transcript, protein, and activity levels. This increase was enhanced by high pressure, which also increased TIMP-2 protein expression despite decreased levels of the cognate transcript. In contrast, the expression of plasminogen activator inhibitor-1 increased with shear stress but was not modified by pressure. Levels of the venous marker Eph-B4 were decreased under arterial shear stress, and levels of the arterial marker Ephrin-B2 were downregulated under high-pressure conditions.
CONCLUSIONS: This model is a valuable tool to identify the role of hemodynamic forces and to decipher the molecular mechanisms leading to failure of human saphenous vein grafts. Under ex vivo conditions, arterial perfusion is sufficient to activate the remodeling of human veins, a change that is associated with the loss of specific vein markers. Elevation of pressure generates intimal hyperplasia, even though veins do not acquire arterial markers.
CLINICAL RELEVANCE: The pathological remodeling of the venous wall, which leads to stenosis and ultimately graft failure, is the main limiting factor of human saphenous vein graft bypass. This remodeling is due to the hemodynamic adaptation of the vein to the arterial environment and cannot be prevented by conventional therapy. To develop a more targeted therapy, a better understanding of the molecular mechanisms involved in intimal hyperplasia is essential, which requires the development of ex vivo models of chronic perfusion of human veins.
Keywords
Aged, Aged, 80 and over, Arterial Pressure, Biomechanics, Cell Proliferation, Ephrin-B2/genetics, Ephrin-B2/metabolism, Female, Gene Expression Regulation, Hemodynamics, Humans, Hyperplasia, Male, Matrix Metalloproteinase 2/genetics, Matrix Metalloproteinase 2/metabolism, Matrix Metalloproteinase 9/genetics, Matrix Metalloproteinase 9/metabolism, Middle Aged, Neointima, Perfusion, Plasminogen Activator Inhibitor 1/genetics, Plasminogen Activator Inhibitor 1/metabolism, Pulsatile Flow, RNA, Messenger/metabolism, Receptor, EphB4/genetics, Receptor, EphB4/metabolism, Saphenous Vein/metabolism, Saphenous Vein/pathology, Stress, Mechanical, Time Factors, Tissue Culture Techniques, Tissue Inhibitor of Metalloproteinase-1/genetics, Tissue Inhibitor of Metalloproteinase-1/metabolism, Tissue Inhibitor of Metalloproteinase-2/genetics, Tissue Inhibitor of Metalloproteinase-2/metabolism, Vascular Patency
Pubmed
Web of science
Open Access
Yes
Create date
11/02/2013 13:58
Last modification date
20/08/2019 15:09
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