Variation of bcl-2 expression in breast ducts and lobules in relation to plasma progesterone levels: overexpression and absence of variation in fibroadenomas.

Details

Serval ID
serval:BIB_A407AEAF62DB
Type
Article: article from journal or magazin.
Collection
Publications
Title
Variation of bcl-2 expression in breast ducts and lobules in relation to plasma progesterone levels: overexpression and absence of variation in fibroadenomas.
Journal
Journal of Pathology
Author(s)
Ferrières G., Cuny M., Simony-Lafontaine J., Jacquemier J., Rouleau C., Guilleux F., Grenier J., Rouanet P., Pujol H., Jeanteur P., Escot C.
ISSN
0022-3417 (Print)
ISSN-L
0022-3417
Publication state
Published
Issued date
1997
Volume
183
Number
2
Pages
204-211
Language
english
Abstract
Some women with benign breast disease eventually develop breast cancer. The mammary gland undergoes tissue remodelling according to hormonal influences, involving a balance between quiescence, proliferation, and mechanisms of cell death. Proliferation and/or apoptotic events could therefore be investigated to help understand the mechanisms of benign lesion formation and identify mastopathies with a poor prognosis. bcl-2 expression was analysed by immunohistochemistry in 75 benign mastopathies. Protein levels were quantitated with an image analyser in various epithelial structures on frozen sections, including adenoses, fibroadenomas, ductal epithelial hyperplasias, cysts, and apparently normal surrounding lobules and ducts. bcl-2 levels were equivalent in apparently normal lobules and ducts, as well as in cysts and ductal hyperplasias. bcl-2 staining was significantly higher in fibroadenomas, known to be of lobular origin [mean = 10.1, quantitative immunochemistry score (QIC) arbitrary units (AU), n = 19], than in normal lobules (mean = 5.1 AU, n = 43, P = 7 x 10(-5). bcl-2 levels in normal lobules and ducts varied according to the menstrual cycle, being higher during the follicular than the luteal phase (P = 1.8 x 10(-2) and P = 1.7 x 10(-2), respectively). This was further supported by a statistical link (P = 5 x 10(-3) between high levels of circulating progesterone and weak bcl-2 staining in lobules and ducts. This progesterone-dependent variation was absent in fibroadenomas. No statistical correlation was found between bcl-2 expression and circulating levels of oestradiol, and follicle-stimulating or luteotrophic hormones. Although these are only preliminary results, they suggest an influence of progesterone on bcl-2 expression which might be lost in fibroadenomas. A hypothesis is proposed concerning the potential involvement of altered regulation of the apoptotic process in the formation of such benign lesions.
Keywords
Breast Neoplasms/metabolism, Breast Neoplasms/pathology, Female, Fibroadenoma/metabolism, Fibroadenoma/pathology, Gonadal Steroid Hormones/blood, Humans, Immunoenzyme Techniques, Menstrual Cycle/metabolism, Neoplasm Proteins/metabolism, Progesterone/blood, Proto-Oncogene Proteins c-bcl-2/metabolism
Pubmed
Create date
11/10/2011 10:33
Last modification date
20/08/2019 15:09
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