Palytoxin effects through interaction with the Na,K-ATPase in Xenopus oocyte

Details

Serval ID
serval:BIB_A3E25FDF6972
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Palytoxin effects through interaction with the Na,K-ATPase in Xenopus oocyte
Journal
FEBS Letters
Author(s)
Wang  X., Horisberger  J. D.
ISSN
0014-5793 (Print)
Publication state
Published
Issued date
06/1997
Volume
409
Number
3
Pages
391-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 16
Abstract
Palytoxin (PTX) is known to bind to Na,K-ATPase, to inhibit its activity, and to induce cation conductance, but the mechanism of these effects is still poorly understood. In Xenopus oocytes, PTX induced a large cation conductance, an effect that could be prevented or reversed by ouabain for oocytes expressing Xenopus Na,K-pumps but not with those expressing Bufo Na,K-pumps. In both cases patch-clamp experiments demonstrated a 7-8 pS channel in the presence of PTX. A large PTX-induced conductance could be observed with minimal Na,K-pump inhibition. From the single PTX-induced channel and macroscopic whole oocyte conductance, and the number of Na,K-pumps, we can conclude that PTX-induced conductance occurs through a direct interaction of PTX with a small number of Na,K-pumps.
Keywords
Acrylamides/metabolism/*pharmacology Animals Bufonidae Cnidarian Venoms/enzymology/*pharmacology Electric Conductivity Kinetics Na(+)-K(+)-Exchanging ATPase/antagonists & inhibitors/drug effects/*metabolism Oocytes/*drug effects/*enzymology Patch-Clamp Techniques Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 12:38
Last modification date
20/08/2019 15:09
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