Analysis of CpG suppression in methylated and nonmethylated species
Details
Serval ID
serval:BIB_A36517BDF755
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of CpG suppression in methylated and nonmethylated species
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
02/1992
Volume
89
Number
3
Pages
957-61
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb 1
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb 1
Abstract
The development of nearest-neighbor analysis led to the finding that the frequency of the dinucleotide CpG is markedly depressed in vertebrates. One explanation of this suppression is that methylation of CpG found in vertebrates represents a mutational hot spot through deamination of methylcytidine to thymidine. We have examined the role of methylated CpG as a factor in CpG suppression by comparing CpG distributions in coding regions of 121 genes from six species, three with methylated DNA and three with nonmethylated DNA. Overall base composition shows that all species exhibit CpG suppression, with the methylated forms showing significantly greater suppression than nonmethylated forms. When the data are analyzed by CpG position, the mean values of the methylated forms exhibit greater suppression than nonmethylated forms at positions I-II and II-III, but there is considerable overlap of suppression scores for individual species. At position III-I, CpG suppression is marked in all methylated species, and it is reversed in all nonmethylated species. Our analysis supports the hypothesis that CpG patterns at positions II-III and III-I in methylated forms are affected by mutation acting through deamination of methylcytidine to thymidine. We speculate that the excess of CpGs at position III-I in nonmethylated forms may be related to a requirement for minimal thermal stability of the DNA.
Keywords
Animals
Arginase/genetics
Base Sequence
Caenorhabditis/genetics
Codon
DNA/chemistry/*genetics
Drosophila melanogaster/genetics
Genes
Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
Humans
Methylation
Mice
Molecular Sequence Data
Phosphoglycerate Kinase/genetics
Plasmodium falciparum/genetics
Saccharomyces cerevisiae/genetics
alpha-Amylase/genetics
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 13:58
Last modification date
20/08/2019 16:09