Protein-binding microarrays: probing disease markers at the interface of proteomics and genomics.

Details

Serval ID
serval:BIB_A3424A57C803
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Protein-binding microarrays: probing disease markers at the interface of proteomics and genomics.
Journal
Trends in Molecular Medicine
Author(s)
Kerschgens J., Egener-Kuhn T., Mermod N.
ISSN
1471-499X[electronic], 1471-4914[linking]
Publication state
Published
Issued date
2009
Volume
15
Number
8
Pages
352-358
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Abstract
DNA-binding proteins mediate a variety of crucial molecular functions, such as transcriptional regulation and chromosome maintenance, replication and repair, which in turn control cell division and differentiation. The roles of these proteins in disease are currently being investigated using microarray-based approaches. However, these assays can be difficult to adapt to routine diagnosis of complex diseases such as cancer. Here, we review promising alternative approaches involving protein-binding microarrays (PBMs) that probe the interaction of proteins from crude cell or tissue extracts with large collections of synthetic or natural DNA sequences. Recent studies have demonstrated the use of these novel PBM approaches to provide rapid and unbiased characterization of DNA-binding proteins as molecular markers of disease, for example cancer progression or infectious diseases.
Keywords
Animals, Biological Markers/chemistry, Communicable Diseases/genetics, Communicable Diseases/metabolism, DNA/genetics, DNA/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Genomics, Humans, Neoplasms/genetics, Neoplasms/metabolism, Protein Array Analysis/methods, Protein Binding, Proteomics
Pubmed
Web of science
Create date
07/10/2009 16:11
Last modification date
20/08/2019 16:08
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