Alternative splicing of type II procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF-beta1.
Details
Serval ID
serval:BIB_A31A1372A511
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Alternative splicing of type II procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF-beta1.
Journal
Febs Letters
ISSN
0014-5793 (Print)
ISSN-L
0014-5793
Publication state
Published
Issued date
2003
Volume
545
Number
2-3
Pages
115-119
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Type II collagen is the major protein of cartilage and is synthesized as a procollagen in two forms (IIA and IIB), generated by differential splicing of the gene primary transcript. Previous studies have indicated that only type IIB is expressed in differentiated chondrocytes. Here, we examined the effects of bone morphogenetic protein (BMP)-2 and transforming growth factor (TGF)-beta1 on the expression of IIA and IIB forms expressed in de-differentiated chondrocytes grown in monolayer. Our results demonstrate that BMP-2 favors expression of type IIB whereas TGF-beta1 potentiates expression of type IIA induced by subculture. These observations reveal the specific capability of BMP-2 to reverse the de-differentiation state of chondrocytes.
Keywords
Alternative Splicing/genetics, Animals, Bone Morphogenetic Protein 2, Bone Morphogenetic Proteins/metabolism, Cell Differentiation/genetics, Cells, Cultured, Chondrocytes/cytology, Chondrocytes/metabolism, Gene Expression Regulation, Developmental, Mice, Procollagen/genetics, Procollagen/metabolism, Protein Isoforms/genetics, Protein Isoforms/metabolism, RNA Precursors/genetics, RNA Precursors/metabolism, Transforming Growth Factor beta/metabolism
Pubmed
Create date
01/10/2015 16:23
Last modification date
20/08/2019 16:08
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