PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.

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Ressource 1Download: Nguyen 2020.pdf (1122.33 [Ko])
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Serval ID
serval:BIB_A26C81C4062D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.
Journal
Plant physiology
Author(s)
Nguyen NNT, Clua J., Vetal P.V., Vuarambon D.J., De Bellis D., Pervent M., Lepetit M., Udvardi M., Valentine A.J., Poirier Y.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Publication state
Published
Issued date
25/02/2021
Peer-reviewed
Oui
Volume
185
Number
1
Pages
196-209
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Legumes play an important role in the soil nitrogen availability via symbiotic nitrogen fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of the high Pi requirement of symbiosis. Whereas PHT1 transporters are involved in Pi uptake into nodules, it is unknown how Pi is transferred from the plant infected cells to nitrogen-fixing bacteroids. We hypothesized that Medicago truncatula genes homologous to Arabidopsis PHO1, encoding a vascular apoplastic Pi exporter, are involved in Pi transfer to bacteroids. Among the seven MtPHO1 genes present in M. truncatula, we found that two genes, namely MtPHO1.1 and MtPHO1.2, were broadly expressed across the various nodule zones in addition to the root vascular system. Expressions of MtPHO1.1 and MtPHO1.2 in Nicotiana benthamiana mediated specific Pi export. Plants with nodule-specific downregulation of both MtPHO1.1 and MtPHO1.2 were generated by RNA interference (RNAi) to examine their roles in nodule Pi homeostasis. Nodules of RNAi plants had lower Pi content and a three-fold reduction in SNF, resulting in reduced shoot growth. Whereas the rate of 33Pi uptake into nodules of RNAi plants was similar to control, transfer of 33Pi from nodule cells into bacteroids was reduced and bacteroids activated their Pi-deficiency response. Our results implicate plant MtPHO1 genes in bacteroid Pi homeostasis and SNF via the transfer of Pi from nodule infected cells to bacteroids.
Pubmed
Open Access
Yes
Create date
17/12/2020 13:49
Last modification date
10/03/2021 7:27
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