Simultaneous determination of mefloquine and chloroquine in biological fluids using high-performance liquid chromatography.
Details
Serval ID
serval:BIB_A205AA84B14B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Simultaneous determination of mefloquine and chloroquine in biological fluids using high-performance liquid chromatography.
Journal
Annales De Biologie Clinique
ISSN
0003-3898[print], 0003-3898[linking]
Publication state
Published
Issued date
1988
Volume
46
Number
9
Pages
722-724
Language
english
Abstract
A high-performance liquid chromatography (HPLC) method for the simultaneous determination of mefloquine and chloroquine in biological fluids is described. Methaqualone is used as an internal standard. After a single-step extraction, achieved from alkalinized samples with diethyl ether, a separation is obtained using a mu-Bondapak phenyl column with a mobile phase of acetonitrile and sodium acetate buffer. Detection is done at 305 nm. The lower detection limits are 3 and 10 micrograms/l for mefloquine and chloroquine, respectively. Thus, this method appears to be simple, rapid, very sensitive and applicable in pharmaco-clinical, toxicological and forensic analyses.
Keywords
Antimalarials/blood, Chloroquine/blood, Chromatography, High Pressure Liquid/methods, Humans, Mefloquine, Methaqualone/blood, Quinolines/blood
Pubmed
Create date
11/10/2010 15:58
Last modification date
20/08/2019 16:08
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