Differences in adult excretory-secretory products between geographical isolates of Echinostoma caproni

Details

Serval ID
serval:BIB_A1ED4F51C96C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differences in adult excretory-secretory products between geographical isolates of Echinostoma caproni
Journal
Journal of Parasitology
Author(s)
Trouve  S., Coustau  C.
ISSN
0022-3395 (Print)
Publication state
Published
Issued date
10/1998
Volume
84
Number
5
Pages
1062-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
Because of the potentially important role of parasite excretory-secretory (ES) products in regulating intermate relationships and host-parasite interactions, we investigated intraspecific variability of adult ES products from a trematode species. Adults from 3 geographical isolates of Echinostoma caproni were collected and maintained under in vitro conditions. ES products were collected at 4, 8, and 22 hr of in vitro maintenance. In order to test for interspecific variability, ES products from a different echinostome species (Echinostoma sp.) were collected in a similar way. Major ES polypeptides were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and silver staining. The polypeptide patterns of total ES products appeared very stable in time for each isolate. ES products from Echinostoma sp. showed a distinct polypeptide profile with none of the major bands being shared with the E. caproni isolates. Although polypeptide patterns from the 3 E. caproni isolates shared most major bands, isolate-specific bands could be observed. Two isolates exhibited a band at 85 and 119 kDa, respectively, whereas the third isolate was characterized by the absence of both bands. These results, together with the previously reported role of ES products in molecular signaling raise the question of the importance of intraspecific ES product differences in evolutionary processes such as assortative mating or local host adaptation.
Keywords
Animals Antigens, Helminth/analysis/chemistry Antigens, Surface/analysis/chemistry Biomphalaria Bulinus Cricetinae Echinostoma/*metabolism Electrophoresis, Polyacrylamide Gel Helminth Proteins/analysis/chemistry Host-Parasite Relations Mice Molecular Weight Peptides/*analysis/chemistry Silver Staining
Pubmed
Web of science
Create date
24/01/2008 18:34
Last modification date
20/08/2019 16:07
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