Plasmacytoid Dendritic Cells Are Productively Infected and Activated through TLR-7 Early after Arenavirus Infection.

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Serval ID
serval:BIB_A192F5A4B96E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Plasmacytoid Dendritic Cells Are Productively Infected and Activated through TLR-7 Early after Arenavirus Infection.
Journal
Cell Host and Microbe
Author(s)
Macal M., Lewis G.M., Kunz S., Flavell R., Harker J.A., Zúñiga E.I.
ISSN
1934-6069 (Electronic)
ISSN-L
1931-3128
Publication state
Published
Issued date
2012
Volume
11
Number
6
Pages
617-630
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish. Author contributions: M.M. designed, performed, analyzed, and interpreted all experiments described; G.M.L helped design, perform, and interpret antigen-presentation assays; S.K. contributed to the design, interpretation, and performance of virus binding assays; R.F. generated the TLR7 / mice; J.A.H. helped design, perform, and interpret the immunofluorescence microscopy; E.I.Z. helped perform initial experiments and oversaw the design, analysis, and interpretation of all studies described. M.M. and E.I.Z. wrote the manuscript.
Abstract
The antiviral response is largely mediated by dendritic cells (DCs), including conventional (c) DCs that function as antigen-presenting cells, and plasmacytoid (p) DCs that produce type I interferons, making them an attractive target for viruses. We find that the Old World arenaviruses lymphocytic choriomeningitis virus clone 13 (LCMV Cl13) and Lassa virus bind pDCs to a greater extent than cDCs. Consistently, LCMV Cl13 targets pDCs early after in vivo infection of its natural murine host and establishes a productive and robust replication cycle. pDCs coproduce type I interferons and proinflammatory cytokines, with the former being induced in both infected and uninfected pDCs, demonstrating a dissociation from intrinsic virus replication. TLR7 globally mediates pDC responses, limits pDC viral load, and promotes rapid innate and adaptive immune cell activation. These early events likely help dictate the outcome of infections with arenaviruses and other DC-replicating viruses and shed light on potential therapeutic targets.
Pubmed
Web of science
Open Access
Yes
Create date
23/07/2012 9:45
Last modification date
20/08/2019 15:07
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