Exposure to kainic acid mimics the effects of axotomy in cerebellar Purkinje cells of the adult rat

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Serval ID
serval:BIB_A15C347D2029
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Exposure to kainic acid mimics the effects of axotomy in cerebellar Purkinje cells of the adult rat
Journal
European Journal of Neuroscience
Author(s)
Rossi  F., Borsello  T., Strata  P.
ISSN
0953-816X (Print)
Publication state
Published
Issued date
03/1994
Volume
6
Number
3
Pages
392-402
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 1
Abstract
We have investigated the long-term structural changes which affect Purkinje cells exposed to a single dose of kainic acid. Following intraparenchymal injection of the excitotoxin in the cerebellar cortex (1 microliter of a 1 mg/ml solution), Purkinje cells which survived within the lesioned area or close to its edges showed remarkable axonal abnormalities, involving the formation of torpedoes, hypertrophy of recurrent collaterals and atrophy of the corticofugal portion of the axon. In addition, their dendritic trees were often affected by conspicuous regressive alterations. The climbing fibres contacting these Purkinje cells were characterized by thick perisomatic plexuses, whereas their peridendritic branches were atrophic. The dendrites innervated by such atrophic olivary arbours were studded with huge numbers of newly formed spines. These alterations were already present a few days after kainic acid administration and persisted for the total period of observation of 6 months after the lesion. The remarkable similarity between the abnormalities of Purkinje cells exposed to kainic acid and those observed after axotomy indicates that in these two conditions common mechanisms determine analogous long-lasting modifications in the affected neurons. It is proposed that kainic acid-induced intracellular calcium overload disrupts cytoskeletal components and impairs axonal transport, thus depriving the affected Purkinje cells of retrograde trophic influences from their target neurons. As a consequence the affected neurons undergo long-lasting regressive modifications and compensatory remodelling phenomena.
Keywords
Animals Axons/*physiology Female Kainic Acid/*pharmacology Male Nerve Fibers/drug effects Propidium/pharmacology Purkinje Cells/*drug effects/ultrastructure Rats Rats, Wistar
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Web of science
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24/01/2008 14:20
Last modification date
20/08/2019 15:07
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