Structural and functional characterization of complement C8 gamma, a member of the lipocalin protein family

Details

Serval ID
serval:BIB_A0D2F301320E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Structural and functional characterization of complement C8 gamma, a member of the lipocalin protein family
Journal
Molecular Immunology
Author(s)
Haefliger  J. A., Peitsch  M. C., Jenne  D. E., Tschopp  J.
ISSN
0161-5890 (Print)
Publication state
Published
Issued date
02/1991
Volume
28
Number
1-2
Pages
123-31
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan-Feb
Abstract
Human complement component C8 exhibits an unusual structure in that it contains three chains, two of which, alpha and beta, display high sequence homology to other complement and CTL pore-forming proteins. The third chain, C8 gamma, is covalently linked to C8 alpha by a disulfide linkage; it is demonstrated that Cys40 of C8 gamma is linked to Cys164 of C8 alpha, a unique cysteine located in a loop located between the cysteine-rich LDL-receptor class A module and the membrane-inserting region of C8 alpha. C8 gamma was recently identified as a member of the lipocalin protein family, in which all proteins were either shown to, or are believed to bind small hydrophobic ligands. The present results now demonstrate that C8 gamma incorporates retinol and retinoic acid in the presence of 2 M NaCl. Molecular modeling of C8 gamma, based on the crystal structure of the homologous beta-lactoglobulin, reveals a structure of eight antiparallel beta-strands, bearing a highly hydrophobic binding pocket. The residues participating in the pocket formation are highly conserved when compared with the structures of beta-lactoglobulin and retinol-binding protein, both of which are known to interact with retinol. It is therefore proposed that C8 gamma may act as a retinol transporting protein in plasma.
Keywords
Alpha-Globulins/chemistry Amino Acid Sequence Complement C8/*chemistry/metabolism Cyanogen Bromide/chemistry Disulfides/chemistry Humans Lactoglobulins/chemistry Ligands Models, Molecular Molecular Sequence Data Multigene Family Peptide Fragments/chemistry Retinol-Binding Proteins/chemistry Solvents Tretinoin/metabolism Vitamin A/metabolism
Pubmed
Web of science
Create date
24/01/2008 16:19
Last modification date
20/08/2019 16:06
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