Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation.

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State: Public
Version: Final published version
Serval ID
serval:BIB_A067B4F4ADC7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation.
Journal
Scientific reports
Author(s)
Beaume M., Köhler T., Greub G., Manuel O., Aubert J.D., Baerlocher L., Farinelli L., Buckling A., van Delden C.
Working group(s)
Swiss Transplant Cohort Study
Contributor(s)
Achermann R., Amico P., Baumann P., Beldi G., Benden C., Berger C., Binet I., Bochud P.Y., Boely E., Bucher H., Bühler L., Carell T., Catana E., Chalandon Y., Geest S., Rougemont O., Dickenmann M., Duchosal M., Fehr T., Ferrari-Lacraz S., Garzoni C., Soccal P.G., Giostra E., Golshayan D., Good D., Hadaya K., Halter J., Heim D., Hess C., Hillinger S., Hirsch H.H., Hofbauer G., Huynh-Do U., Immer F., Klaghofer R., Koller M., Laesser B., Lehmann R., Lovis C., Marti H.P., Martin P.Y., Martinolli L., Meylan P., Mohacsi P., Morard I., Morel P., Mueller U., Mueller N.J., Mueller-McKenna H., Müller A., Müller T., Müllhaupt B., Nadal D., Pascual M., Passweg J., Ziegler C.P., Rick J., Roosnek E., Rosselet A., Rothlin S., Ruschitzka F., Schanz U., Schaub S., Seiler C., Stampf S., Steiger J., Stirnimann G., Toso C., Tsinalis D., Venetz J.P., Villard J., Wick M., Wilhelm M., Yerly P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
17/01/2017
Peer-reviewed
Oui
Volume
7
Pages
40309
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
In cystic fibrosis (CF) patients, chronic airway infection by Pseudomonas leads to progressive lung destruction ultimately requiring lung transplantation (LT). Following LT, CF-adapted Pseudomonas strains, potentially originating from the sinuses, may seed the allograft leading to infections and reduced allograft survival. We investigated whether CF-adapted Pseudomonas populations invade the donor microbiota and adapt to the non-CF allograft. We collected sequential Pseudomonas isolates and airway samples from a CF-lung transplant recipient during two years, and followed the dynamics of the microbiota and Pseudomonas populations. We show that Pseudomonas invaded the host microbiota within three days post-LT, in association with a reduction in richness and diversity. A dominant mucoid and hypermutator mutL lineage was replaced after 11 days by non-mucoid strains. Despite antibiotic therapy, Pseudomonas dominated the allograft microbiota until day 95. We observed positive selection of pre-LT variants and the appearance of novel mutations. Phenotypic adaptation resulted in increased biofilm formation and swimming motility capacities. Pseudomonas was replaced after 95 days by a microbiota dominated by Actinobacillus. In conclusion, mucoid Pseudomonas adapted to the CF-lung remained able to invade the allograft. Selection of both pre-existing non-mucoid subpopulations and of novel phenotypic traits suggests rapid adaptation of Pseudomonas to the non-CF allograft.
Keywords
Adaptation, Physiological, Adult, Allografts, Colony Count, Microbial, Cystic Fibrosis/microbiology, Female, Genome, Bacterial, Humans, Lung/microbiology, Lung Transplantation, Microbiota, Phenotype, Pseudomonas/isolation & purification, Pseudomonas/physiology, Tissue Donors
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2017 18:04
Last modification date
21/11/2022 8:25
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