HDLs protect pancreatic β-cells against ER stress by restoring protein folding and trafficking.

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State: Public
Version: author
Serval ID
serval:BIB_A0510DB24A81
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
HDLs protect pancreatic β-cells against ER stress by restoring protein folding and trafficking.
Journal
Diabetes
Author(s)
Pétremand J., Puyal J., Chatton J.Y., Duprez J., Allagnat F., Frias M., James R.W., Waeber G., Jonas J.C., Widmann C.
ISSN
1939-327X (Electronic)
ISSN-L
0012-1797
Publication state
Published
Issued date
2012
Volume
61
Number
5
Pages
1100-1111
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Endoplasmic reticulum (ER) homeostasis alteration contributes to pancreatic β-cell dysfunction and death and favors the development of diabetes. In this study, we demonstrate that HDLs protect β-cells against ER stress induced by thapsigargin, cyclopiazonic acid, palmitate, insulin overexpression, and high glucose concentrations. ER stress marker induction and ER morphology disruption mediated by these stimuli were inhibited by HDLs. Using a temperature-sensitive viral glycoprotein folding mutant, we show that HDLs correct impaired protein trafficking and folding induced by thapsigargin and palmitate. The ability of HDLs to protect β-cells against ER stress was inhibited by brefeldin A, an ER to Golgi trafficking blocker. These results indicate that HDLs restore ER homeostasis in response to ER stress, which is required for their ability to promote β-cell survival. This study identifies a cellular mechanism mediating the beneficial effect of HDLs on β-cells against ER stress-inducing factors.
Keywords
Animals, Apoptosis, Cells, Cultured, Endoplasmic Reticulum/physiology, Green Fluorescent Proteins/metabolism, Humans, Insulin-Secreting Cells/cytology, Insulin-Secreting Cells/metabolism, Insulinoma/metabolism, Lipoproteins, HDL/genetics, Lipoproteins, HDL/metabolism, Male, Membrane Proteins/metabolism, Mice, Mutation, Protein Folding, Protein Transport, Rats, Rats, Wistar, Stress, Physiological/physiology, Viral Fusion Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
02/05/2012 14:28
Last modification date
20/08/2019 15:06
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