Chromatographic characterisation of the circulating neuropeptide Y immunoreactivity from patients with phaeochromocytoma

Details

Serval ID
serval:BIB_A02A7045B86B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Chromatographic characterisation of the circulating neuropeptide Y immunoreactivity from patients with phaeochromocytoma
Journal
Regulatory Peptides
Author(s)
Corder  R., Lowry  P. J., Emson  P. C., Gaillard  R. C.
ISSN
0167-0115 (Print)
Publication state
Published
Issued date
03/1985
Volume
10
Number
2-3
Pages
91-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
Samples of adrenal medullary phaeochromocytoma from two patients were extracted in acid. The tissue concentrations of neuropeptide Y (NPY)-like immunoreactivity were found to be 0.94 and 8.11 micrograms/g. Plasma from these patients subjected to NPY radioimmunoassay after Sephadex G-75 gel filtration chromatography revealed circulating plasma concentrations of 1889 and 2079 pg/ml, compared to levels in normal plasma of less than 50 pg/ml, treated in the same way. Further characterisation of this plasma NPY-like immunoreactivity by HPLC showed this peptide to possess identical elution behaviour to human NPY standard. It is suggested that circulating NPY may be a contributory factor in the symptoms exhibited by some patients with adrenal medullary phaeochromocytoma.
Keywords
Adrenal Gland Neoplasms/analysis/*blood Chromatography, Gel Female Humans Male Middle Aged Nerve Tissue Proteins/analysis/*blood Neuropeptide Y Pheochromocytoma/analysis/*blood Radioimmunoassay
Pubmed
Web of science
Create date
15/02/2008 16:58
Last modification date
20/08/2019 15:06
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