Measuring the Activity of Plasma Membrane and Vacuolar Transporters in Yeast

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Version: Author's accepted manuscript
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Serval ID
serval:BIB_A0263920AC22
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Measuring the Activity of Plasma Membrane and Vacuolar Transporters in Yeast
Title of the book
Methods in Molecular Biology
Author(s)
Cools Melody, Rompf Maria, Mayer Andreas, André Bruno
Publisher
Springer New York
ISBN
9781493997350
9781493997367
ISSN
1064-3745
1940-6029
ISSN-L
1064-3745
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
2049
Pages
247-261
Language
english
Abstract
The yeast proteome includes about 300 polytopic membrane proteins known or predicted to function as transporters. Such proteins ensure active or passive transport of small ions or metabolites across the plasma or internal membranes. Despite decades of research on yeast transporters, many of these remain uncharacterized in terms of substrate selectivity range, subcellular localization, and biological function. Assaying the uptake of radiolabeled compounds into whole cells or isolated organelles remains a powerful method for characterizing the function and biochemical properties of these proteins. Here we describe established protocols for measuring transporter activity in whole cells, intact vacuoles, or reconstituted vacuolar vesicles. These methods have proved particularly useful in the context of our work on yeast amino acid transporters, and can in principle be applied to assaying the uptake of other categories of compounds.
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Web of science
Create date
13/10/2019 19:38
Last modification date
29/06/2024 9:30
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