Measuring the Activity of Plasma Membrane and Vacuolar Transporters in Yeast.

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State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_A0263920AC22
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Measuring the Activity of Plasma Membrane and Vacuolar Transporters in Yeast.
Journal
Methods in molecular biology
Author(s)
Cools M., Rompf M., Mayer A., André B.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
2049
Pages
247-261
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The yeast proteome includes about 300 polytopic membrane proteins known or predicted to function as transporters. Such proteins ensure active or passive transport of small ions or metabolites across the plasma or internal membranes. Despite decades of research on yeast transporters, many of these remain uncharacterized in terms of substrate selectivity range, subcellular localization, and biological function. Assaying the uptake of radiolabeled compounds into whole cells or isolated organelles remains a powerful method for characterizing the function and biochemical properties of these proteins. Here we describe established protocols for measuring transporter activity in whole cells, intact vacuoles, or reconstituted vacuolar vesicles. These methods have proved particularly useful in the context of our work on yeast amino acid transporters, and can in principle be applied to assaying the uptake of other categories of compounds.
Keywords
Biological Transport, Cell Membrane/metabolism, Membrane Transport Proteins/metabolism, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/metabolism, Vacuoles/metabolism, Uptake assay, Uptake assay in yeast, Yeast membrane transporter, Yeast vacuole
Pubmed
Create date
13/10/2019 18:38
Last modification date
18/09/2020 5:26
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