Increased renal responsiveness to vasopressin and enhanced V2 receptor signaling in RGS2-/- mice.

Details

Serval ID
serval:BIB_A01914338ED3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Increased renal responsiveness to vasopressin and enhanced V2 receptor signaling in RGS2-/- mice.
Journal
Journal of the American Society of Nephrology
Author(s)
Zuber A.M., Singer D., Penninger J.M., Rossier B.C., Firsov D.
ISSN
1046-6673 (Print)
ISSN-L
1046-6673
Publication state
Published
Issued date
2007
Volume
18
Number
6
Pages
1672-1678
Language
english
Abstract
The antidiuretic effect of vasopressin is mediated by V2 receptors (V2R) that are located in kidney connecting tubules and collecting ducts. This study provides evidence that V2R signaling is negatively regulated by regulator of G protein signaling 2 (RGS2), a member of the family of RGS proteins. This study demonstrates that (1) RGS2 expression in the kidney is restricted to the vasopressin-sensitive part of the nephron (thick ascending limb, connecting tubule, and collecting duct); (2) expression of RGS2 is rapidly upregulated by vasopressin; (3) the vasopressin-dependent accumulation of cAMP, the principal messenger of V2R signaling, is significantly higher in collecting ducts that are microdissected from the RGS2(-/-) mice compared with their wild-type littermates; and (4) analysis of urine output of mice that were exposed to water restriction followed by acute water loading revealed that RGS2(-/-) mice exhibit an increased renal responsiveness to vasopressin. It is proposed that RGS2 is involved in negative feedback regulation of V2R signaling.
Keywords
Animals, Antidiuretic Agents/pharmacology, Cell Line, Cyclic AMP/metabolism, Drinking/physiology, Endothelin-1/pharmacology, Feedback, Physiological/drug effects, Feedback, Physiological/physiology, Kidney Tubules, Collecting/drug effects, Kidney Tubules, Collecting/metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Nephrons/drug effects, Nephrons/metabolism, Phenylephrine/pharmacology, RGS Proteins/genetics, RGS Proteins/metabolism, RNA, Messenger/metabolism, Receptors, Vasopressin/drug effects, Receptors, Vasopressin/metabolism, Signal Transduction/drug effects, Signal Transduction/physiology, Sodium/blood, Sodium/urine, Sympathomimetics/pharmacology, Vasopressins/pharmacology, Water Deprivation/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 12:32
Last modification date
20/08/2019 15:06
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