Arabidopsis H<sup>+</sup>-ATPase AHA1 controls slow wave potential duration and wound-response jasmonate pathway activation.

Details

Serval ID
serval:BIB_9F1340B45C16
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Arabidopsis H<sup>+</sup>-ATPase AHA1 controls slow wave potential duration and wound-response jasmonate pathway activation.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Kumari A., Chételat A., Nguyen C.T., Farmer E.E.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
01/10/2019
Peer-reviewed
Oui
Volume
116
Number
40
Pages
20226-20231
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Electrogenic proton pumps have been implicated in the generation of slow wave potentials (SWPs), damage-induced membrane depolarizations that activate the jasmonate (JA) defense pathway in leaves distal to wounds. However, no defined H <sup>+</sup> -ATPases have been shown to modulate these electrical signals. Pilot experiments revealed that the proton pump activator fusicoccin attenuated SWP duration in Arabidopsis Using mutant analyses, we identified Arabidopsis H <sup>+</sup> -ATPase 1 (AHA1) as a SWP regulator. The duration of the repolarization phase was strongly extended in reduced function aha1 mutants. Moreover, the duration of SWP repolarization was shortened in the presence of a gain-of-function AHA1 allele. We employed aphid electrodes to probe the effects of the aha1 mutation on wound-stimulated electrical activity in the phloem. Relative to the wild type, the aha1-7 mutant increased the duration and reduced the amplitudes of electrical signals in sieve tube cells. In addition to affecting electrical signaling, expression of the JA pathway marker gene JAZ10 in leaves distal to wounds was enhanced in aha1-7 Consistent with this, levels of wound-response jasmonoyl-isoleucine were enhanced in the mutant, as was defense against a lepidopteran herbivore. The work identifies a discrete member of the P-type ATPase superfamily with a role in leaf-to-leaf electrical signaling and plant defense.
Keywords
Arabidopsis/physiology, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Cyclopentanes/metabolism, Electrophysiological Phenomena, Herbivory, Membrane Potentials/genetics, Metabolic Networks and Pathways, Oxylipins/metabolism, Phenotype, Proton Pumps/genetics, Proton Pumps/metabolism, Proton-Translocating ATPases/genetics, Proton-Translocating ATPases/metabolism, Signal Transduction, defense, jasmonate, proton ATPase, vasculature, wound
Pubmed
Web of science
Open Access
Yes
Create date
20/09/2019 22:38
Last modification date
03/04/2020 6:19
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