Determination of temozolomide in human plasma and urine by high-performance liquid chromatography after solid-phase extraction

Details

Serval ID
serval:BIB_9F07B0BF669F
Type
Article: article from journal or magazin.
Collection
Publications
Title
Determination of temozolomide in human plasma and urine by high-performance liquid chromatography after solid-phase extraction
Journal
Journal of Chromatography B, Biomedical Applications
Author(s)
Shen  F., Decosterd  L. A., Gander  M., Leyvraz  S., Biollax  J., Lejeune  F.
ISSN
1572-6495 (Print)
Publication state
Published
Issued date
05/1995
Volume
667
Number
2
Pages
291-300
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 19
Abstract
As a part of a pilot clinical study, a high-performance reversed-phase liquid chromatography analysis was developed to quantify temozolomide in plasma and urine of patients undergoing a chemotherapy cycle with temozolomide. All samples were immediately stabilized with 1 M HCl (1 + 10 of biological sample), frozen and stored at -20 degrees C prior to analysis. The clean-up procedure involved a solid-phase extraction (SPE) of clinical sample (100 microliters) on a 100-mg C18-endcapped cartridge. Matrix components were eliminated with 750 microliters of 0.5% acetic acid (AcOH). Temozolomide was subsequently eluted with 1250 microliters of methanol (MeOH). The resulting eluate was evaporated under nitrogen at RT and reconstituted in 200 microliters of 0.5% AcOH and subjected to HPLC analysis on an ODS-column (MeOH-0.5% AcOH, 10:90) with UV detection at 330 nm. The calibration curves were linear over the concentration range 0.4-20 micrograms/ml and 2-150 micrograms/ml for plasma and urine, respectively. The extraction recovery of temozolomide was 86-90% from plasma and 103-105% from urine over the range of concentrations considered. The stability of temozolomide was studied in vitro in buffered solutions at RT, and in plasma and urine at 37 degrees C. An acidic pH (< 5-6) should be maintained throughout the collection, the processing and the analysis of the sample to preserve the integrity of the drug. The method reported here was validated for use in a clinical study of temozolomide for the treatment of metastatic melanoma and high grade glioma.
Keywords
*Antineoplastic Agents Chromatography, High Pressure Liquid/*methods/statistics & numerical data Dacarbazine/*analogs & derivatives/blood/pharmacokinetics/urine Drug Stability Humans Hydrogen-Ion Concentration Pilot Projects Sensitivity and Specificity
Pubmed
Web of science
Create date
28/01/2008 9:31
Last modification date
20/08/2019 16:05
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