Contribution of IgH-PCR to the evaluation of B-cell lymphoma involvement in paraffin-embedded bone marrow biopsy specimens.

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_9E3350E90881
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Contribution of IgH-PCR to the evaluation of B-cell lymphoma involvement in paraffin-embedded bone marrow biopsy specimens.
Journal
American journal of clinical pathology
Author(s)
Braunschweig R., Baur A.S., Delacrétaz F., Bricod C., Benhattar J.
ISSN
0002-9173 (Print)
ISSN-L
0002-9173
Publication state
Published
Issued date
05/2003
Peer-reviewed
Oui
Volume
119
Number
5
Pages
634-642
Language
english
Notes
Publication types: Evaluation Study ; Journal Article
Publication Status: ppublish
Abstract
We investigated whether the determination of clonality by polymerase chain reaction (PCR) analysis of immunoglobulin heavy chain (IgH) gene rearrangements could be helpful in the evaluation of B-cell lymphoma (BCL) involvement of bone marrow (BM) biopsy specimens. We evaluated 83 paraffin-embedded BM biopsy specimens from 26 patients with BCL. When BM biopsy specimens considered positive, "suspicious," or negative by morphologic and immunohistochemical examination were evaluated by PCR, a monoclonal B-cell population was detected in 81% (39/48), 64% (9/14), and 11% (2/18), respectively. In most cases, a reproducible monoclonal IgH gene rearrangement was observed from BM and extramedullary sites. Nevertheless, in 4 cases, a different and independent monoclonal IgH rearrangement was observed during the disease course. PCR is efficient and complementary to morphologic and immunohistochemical examination for the evaluation of BCL involvement of BM biopsy specimens, especially when a reproducible rearrangement is found in 2 different samples.
Keywords
Amino Acid Sequence, Biopsy, Bone Marrow Examination/methods, Gene Rearrangement, B-Lymphocyte, Heavy Chain, Genetic Heterogeneity, Humans, Immunoglobulin Heavy Chains/genetics, Immunohistochemistry, Lymphoma, B-Cell/pathology, Molecular Sequence Data, Paraffin Embedding, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 18:34
Last modification date
16/07/2020 8:42
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