Evidence for conserved post-transcriptional roles of unitary pseudogenes and for frequent bifunctionality of mRNAs.

Details

Serval ID
serval:BIB_9DECEDBA2898
Type
Article: article from journal or magazin.
Collection
Publications
Title
Evidence for conserved post-transcriptional roles of unitary pseudogenes and for frequent bifunctionality of mRNAs.
Journal
Genome Biology
Author(s)
Marques A.C., Tan J., Lee S., Kong L., Heger A., Ponting C.P.
ISSN
1474-760X (Electronic)
ISSN-L
1474-7596
Publication state
Published
Issued date
2012
Volume
13
Number
11
Pages
R102
Language
english
Abstract
BACKGROUND: Recent reports have highlighted instances of mRNAs that, in addition to coding for protein, regulate the abundance of related transcripts by altering microRNA availability. These two mRNA roles - one mediated by RNA and the other by protein - are inter-dependent and hence cannot easily be separated. Whether the RNA-mediated role of transcripts is important, per se, or whether it is a relatively innocuous consequence of competition by different transcripts for microRNA binding remains unknown.
RESULTS: Here we took advantage of 48 loci that encoded proteins in the earliest eutherian ancestor, but whose protein-coding capability has since been lost specifically during rodent evolution. Sixty-five percent of such loci, which we term 'unitary pseudogenes', have retained their expression in mouse and their transcripts exhibit conserved tissue expression profiles. The maintenance of these unitary pseudogenes' spatial expression profiles is associated with conservation of their microRNA response elements and these appear to preserve the post-transcriptional roles of their protein-coding ancestor. We used mouse Pbcas4, an exemplar of these transcribed unitary pseudogenes, to experimentally test our genome-wide predictions. We demonstrate that the role of Pbcas4 as a competitive endogenous RNA has been conserved and has outlived its ancestral gene's loss of protein-coding potential.
CONCLUSIONS: These results show that post-transcriptional regulation by bifunctional mRNAs can persist over long evolutionary time periods even after their protein coding ability has been lost.
Keywords
Animals, Base Sequence, Cell Line, Conserved Sequence, Dogs, Gene Expression Profiling, Humans, Mice, MicroRNAs/genetics, MicroRNAs/metabolism, Molecular Sequence Data, Phylogeny, Pseudogenes, RNA Processing, Post-Transcriptional, RNA, Messenger/metabolism, Rodentia/genetics
Pubmed
Web of science
Open Access
Yes
Create date
27/10/2014 14:45
Last modification date
20/08/2019 15:04
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