A novel pathway of alloantigen presentation by dendritic cells

Details

Serval ID
serval:BIB_9DB8E76E0C02
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel pathway of alloantigen presentation by dendritic cells
Journal
Journal of Immunology
Author(s)
Herrera  O. B., Golshayan  D., Tibbott  R., Salcido Ochoa  F., James  M. J., Marelli-Berg  F. M., Lechler  R. I.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
10/2004
Volume
173
Number
8
Pages
4828-37
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
In the context of transplantation, dendritic cells (DCs) can sensitize alloreactive T cells via two pathways. The direct pathway is initiated by donor DCs presenting intact donor MHC molecules. The indirect pathway results from recipient DCs processing and presenting donor MHC as peptide. This simple dichotomy suggests that T cells with direct and indirect allospecificity cannot cross-regulate each other because distinct APCs are involved. In this study we describe a third, semidirect pathway of MHC alloantigen presentation by DCs that challenges this conclusion. Mouse DCs, when cocultured with allogeneic DCs or endothelial cells, acquired substantial levels of class I and class II MHC:peptide complexes in a temperature- and energy-dependent manner. Most importantly, DCs acquired allogeneic MHC in vivo upon migration to regional lymph nodes. The acquired MHC molecules were detected by Ab staining and induced proliferation of Ag-specific T cells in vitro. These data suggest that recipient DCs, due to acquisition of donor MHC molecules, may link T cells with direct and indirect allospecificity.
Keywords
Adenosine Triphosphate/metabolism Animals *Antigen Presentation Cells, Cultured Dendritic Cells/*physiology Isoantigens/*immunology Mice Mice, Inbred Strains T-Lymphocytes/immunology Temperature
Pubmed
Web of science
Create date
25/01/2008 13:45
Last modification date
20/08/2019 15:04
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