The membrane attack complex of complement: lipid insertion of tubular and nontubular polymerized C9

Details

Serval ID
serval:BIB_9DA07287B510
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The membrane attack complex of complement: lipid insertion of tubular and nontubular polymerized C9
Journal
Biochemistry
Author(s)
Amiguet  P., Brunner  J., Tschopp  J.
ISSN
0006-2960 (Print)
Publication state
Published
Issued date
12/1985
Volume
24
Number
25
Pages
7328-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 3
Abstract
The membrane-restricted photoactivatable carbene generator 3-(trifluoromethyl)-3-(m-[125I]-iodophenyl)diazirine [Brunner, J., & Semenza, G. (1981) Biochemistry 20, 7174-7182] was used to label the subunits of the membrane attack complex of complement (C5b-9). C5b-9 complexes either were assembled from serum on erythrocyte membranes or were reconstituted from purified components on liposomes. After irradiation, most of the probe is bound to C9 independent of the membrane system used, indicating that the wall of the transmembrane channel is predominantly composed of C9. No difference was observed whether polymerized C9 was in the tubular or nontubular form [Podack, E. R., & Tschopp, J. (1983) J. Biol. Chem. 257, 15204-15212], showing that tubule closure is not essential for successful lipid insertion. The same label distribution between the two forms of polymerized C9 was obtained by analyzing zinc-polymerized C9 in the absence of C5b-8. Since the photoreactive probe reacted with at least two distinct polypeptide segments within C9, lipid interaction does not occur via a single segment of hydrophobic amino acids.
Keywords
Animals Azirines Complement C9/*metabolism Complement Membrane Attack Complex Complement System Proteins/isolation & purification/*metabolism Electrophoresis, Polyacrylamide Gel Erythrocyte Membrane/metabolism Humans *Liposomes Macromolecular Substances Models, Biological Molecular Weight *Phosphatidylcholines Photochemistry Rabbits
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 15:04
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