Specification of haematopoietic stem cell fate via modulation of mitochondrial activity.

Details

Serval ID
serval:BIB_9CF125118D0C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Specification of haematopoietic stem cell fate via modulation of mitochondrial activity.
Journal
Nature Communications
Author(s)
Vannini N., Girotra M., Naveiras O., Nikitin G., Campos V., Giger S., Roch A., Auwerx J., Lutolf M.P.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
7
Pages
13125
Language
english
Notes
Publication types: ARTICLE Publication Status: epublish

Abstract
Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. However, whether this change in the metabolic program is the cause or the consequence of the unique function of HSCs remains unknown. Here we show that enforced modulation of energy metabolism impacts HSC self-renewal. Lowering the mitochondrial activity of HSCs by chemically uncoupling the electron transport chain drives self-renewal under culture conditions that normally induce rapid differentiation. We demonstrate that this metabolic specification of HSC fate occurs through the reversible decrease of mitochondrial mass by autophagy. Our data thus reveal a causal relationship between mitochondrial metabolism and fate choice of HSCs and also provide a valuable tool to expand HSCs outside of their native bone marrow niches.
Pubmed
Web of science
Open Access
Yes
Create date
19/10/2016 11:26
Last modification date
05/08/2020 5:26
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