Deficient signaling in mice devoid of double-stranded RNA-dependent protein kinase.

Details

Serval ID
serval:BIB_9CEF7E93B40B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Deficient signaling in mice devoid of double-stranded RNA-dependent protein kinase.
Journal
EMBO Journal
Author(s)
Yang Y.L., Reis L.F., Pavlovic J., Aguzzi A., Schäfer R., Kumar A., Williams B.R., Aguet M., Weissmann C.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Publication state
Published
Issued date
1995
Volume
14
Number
24
Pages
6095-6106
Language
english
Abstract
Double-stranded RNA-dependent protein kinase (PKR) has been implicated in interferon (IFN) induction, antiviral response and tumor suppression. We have generated mice devoid of functional PKR (Pkr%). Although the mice are physically normal and the induction of type I IFN genes by poly(I).poly(C) (pIC) and virus is unimpaired, the antiviral response induced by IFN-gamma and pIC was diminished. However, in embryo fibroblasts from Pkr knockout mice, the induction of type I IFN as well as the activation of NF-kappa B by pIC, were strongly impaired but restored by priming with IFN. Thus, PKR is not directly essential for responses to pIC, and a pIC-responsive system independent of PKR is induced by IFN. No evidence of the tumor suppressor activity of PKR was demonstrated.
Keywords
Animals, Base Sequence, Cell Transformation, Neoplastic, Cells, Cultured, DNA Primers/genetics, Encephalomyocarditis virus/pathogenicity, Gene Expression Regulation/drug effects, Interferon Type I/genetics, Interferons/pharmacology, Mice, Mice, Knockout, Molecular Sequence Data, NF-kappa B/metabolism, Poly I-C/pharmacology, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/physiology, Signal Transduction, eIF-2 Kinase
Pubmed
Web of science
Create date
28/01/2008 11:37
Last modification date
20/08/2019 15:03
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