Isolation of oxalotrophic bacteria able to disperse on fungal mycelium

Details

Ressource 1Download: file (850.83 [Ko])
State: Public
Version: Final published version
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_9CB6AB8B1C9E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of oxalotrophic bacteria able to disperse on fungal mycelium
Journal
FEMS MICROBIOLOGY LETTERS
Author(s)
Bravo Daniel, Cailleau Guillaume, Bindschedler Saskia, Simon Anaele, Job Daniel, Verrecchia Eric, Junier Pilar
ISSN-L
0378-1097
Publication state
Published
Issued date
11/2013
Volume
348
Number
2
Pages
157-166
Language
english
Notes
ISI:000326056800010
Abstract
A technique based on an inverted Petri dish system was developed for the growth and isolation of soil oxalotrophic bacteria able to disperse on fungal mycelia. The method is related to the fungal highways' dispersion theory in which mycelial fungal networks allow active movement of bacteria in soil. Quantification of this phenomenon showed that bacterial dispersal occurs preferentially in upper soil horizons. Eight bacteria and one fungal strain were isolated by this method. The oxalotrophic activity of the isolated bacteria was confirmed through calcium oxalate dissolution in solid selective medium. After separation of the bacteria-fungus couple, partial sequencing of the 16S and the ITS1 and ITS2 sequences of the ribosomal RNA genes were used for the identification of bacteria and the associated fungus. The isolated oxalotrophic bacteria included strains related to Stenotrophomonas, Achromobacter, Lysobacter, Pseudomonas, Agrobacterium, Cohnella, and Variovorax. The recovered fungus corresponded to Trichoderma sp. A test carried out to verify bacterial transport in an unsaturated medium showed that all the isolated bacteria were able to migrate on Trichoderma hyphae or glass fibers to re-colonize an oxalate-rich medium. The results highlight the importance of fungus-driven bacterial dispersal to understand the functional role of oxalotrophic bacteria andfungi in soils.
Open Access
Yes
Create date
17/02/2014 13:47
Last modification date
08/12/2021 7:38
Usage data