Isolation of oxalotrophic bacteria able to disperse on fungal mycelium

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Serval ID
serval:BIB_9CB6AB8B1C9E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of oxalotrophic bacteria able to disperse on fungal mycelium
Journal
FEMS MICROBIOLOGY LETTERS
Author(s)
Bravo Daniel, Cailleau Guillaume, Bindschedler Saskia, Simon Anaele, Job Daniel, Verrecchia Eric, Junier Pilar
ISSN-L
0378-1097
Publication state
Published
Issued date
11/2013
Volume
348
Number
2
Pages
157-166
Notes
ISI:000326056800010
Abstract
A technique based on an inverted Petri dish system was developed for the
growth and isolation of soil oxalotrophic bacteria able to disperse on
fungal mycelia. The method is related to the fungal highways' dispersion
theory in which mycelial fungal networks allow active movement of
bacteria in soil. Quantification of this phenomenon showed that
bacterial dispersal occurs preferentially in upper soil horizons. Eight
bacteria and one fungal strain were isolated by this method. The
oxalotrophic activity of the isolated bacteria was confirmed through
calcium oxalate dissolution in solid selective medium. After separation
of the bacteria-fungus couple, partial sequencing of the 16S and the
ITS1 and ITS2 sequences of the ribosomal RNA genes were used for the
identification of bacteria and the associated fungus. The isolated
oxalotrophic bacteria included strains related to Stenotrophomonas,
Achromobacter, Lysobacter, Pseudomonas, Agrobacterium, Cohnella, and
Variovorax. The recovered fungus corresponded to Trichoderma sp. A test
carried out to verify bacterial transport in an unsaturated medium
showed that all the isolated bacteria were able to migrate on
Trichoderma hyphae or glass fibers to re-colonize an oxalate-rich
medium. The results highlight the importance of fungus-driven bacterial
dispersal to understand the functional role of oxalotrophic bacteria and
fungi in soils.
Open Access
Yes
Create date
17/02/2014 12:47
Last modification date
25/09/2019 6:10
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