Kainate-induced endocytosis in retinal amacrine cells.

Details

Serval ID
serval:BIB_9CB402743636
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Kainate-induced endocytosis in retinal amacrine cells.
Journal
Journal of Comparative Neurology
Author(s)
Borsello T., Bressoud R., Mottier V., González N., Gomez G., Clarke P.G.H.
ISSN
0021-9967[print], 0021-9967[linking]
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
465
Number
2
Pages
286-295
Language
english
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Oct 13
Abstract
Endocytosis is enhanced in some cases of neuronal death. We report for the first time that intraocular injections, in chick embryos, of excitotoxic doses of kainate induce strong endocytosis in retinal amacrine cells destined to die and that even subtoxic doses can induce some degree of endocytosis. That the uptake was due to endocytosis rather than passive diffusion through the plasma membrane was shown ultrastructurally. The endocytosis was demonstrated by using three unrelated tracers--horseradish peroxidase, microperoxidase, and 4.4-kDa fluorescein isothiocyanate (FITC)-labeled dextran--suggesting that it does not depend on the binding of the tracers to a particular receptor. However, it appears to be surprisingly sensitive to the size of the ligand, because a heavier (42-kDa) FITC-dextran was not endocytosed. The induction of endocytosis by kainate can occur even when protein synthesis is blocked. These results indicate that toxic or near-toxic doses of kainate induce endocytosis, raising the question of whether this is mechanistically implicated in causing or preventing excitotoxic neuronal death.
Keywords
Amacrine Cells/drug effects, Amacrine Cells/metabolism, Animals, Cell Count, Chick Embryo, Cycloheximide/pharmacology, DNA Fragmentation/drug effects, Dose-Response Relationship, Drug, Endocytosis/drug effects, Excitatory Amino Acid Agonists/pharmacology, Fluorescent Antibody Technique, In Situ Nick-End Labeling, Kainic Acid/pharmacology, Microscopy, Electron, Protein Synthesis Inhibitors/pharmacology
Pubmed
Web of science
Create date
24/01/2008 14:19
Last modification date
20/08/2019 15:03
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