Tissue-specific chromatin structure at the hepatocyte growth factor/scatter factor gene promoter

Details

Serval ID
serval:BIB_9CB0963CB63B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Tissue-specific chromatin structure at the hepatocyte growth factor/scatter factor gene promoter
Journal
Gene
Author(s)
Price  M., Fivaz  J., Jotterand  A., Mirkovitch  J.
ISSN
0378-1119 (Print)
Publication state
Published
Issued date
04/1998
Volume
211
Number
1
Pages
141-50
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 28
Abstract
Hepatocyte growth factor/scatter factor (HGF/SF) is a recently characterised molecule with many remarkable functions. Its involvement in important processes such as cell proliferation, cell migration, morphogenesis and organ development implies that its activity should be tightly regulated. To understand the molecular mechanisms controlling HGF/SF transcription, we have analysed DNaseI hypersensitive sites (DHS) along rat and human HGF/SF genes in various tissues and cell types. We identified five DHS along the rat gene, two in the 5'-flanking region and three in the first intron. These sites are only found in rat tissues and rat cell lines, which express HGF/SF. The strongest hypersensitive site map to a region that corresponds to the promoter by start site analysis. A single tissue-specific DHS is present in human cell lines that express HGF/SF and corresponds to the promoter region. Our results suggest that chromatin accessibility plays a major role in the regulation of HGF/SF transcription regulation.
Keywords
Animals Cell Line Chromatin/*genetics DNA/metabolism Deoxyribonuclease I/metabolism Hepatocyte Growth Factor/*genetics Humans Interleukin-3/*chemistry Micrococcal Nuclease/metabolism Promoter Regions (Genetics)/*genetics RNA, Messenger/metabolism Rats Transcription, Genetic/genetics
Pubmed
Web of science
Create date
25/01/2008 8:41
Last modification date
20/08/2019 15:03
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