Rapid Polymyxin/Pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_9CACBEB7AEE9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid Polymyxin/Pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa.
Journal
European journal of clinical microbiology & infectious diseases
Author(s)
Sadek M., Tinguely C., Poirel L., Nordmann P.
ISSN
1435-4373 (Electronic)
ISSN-L
0934-9723
Publication state
Published
Issued date
09/2020
Peer-reviewed
Oui
Volume
39
Number
9
Pages
1657-1662
Language
english
Notes
Publication types: Evaluation Study ; Journal Article
Publication Status: ppublish
Abstract
Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually detected by yellow to purple/violet color change of the bromocresol purple pH indicator. The test performance was evaluated by using 50 colistin-resistant and colistin-susceptible P. aeruginosa isolates, among which 10 were colistin resistant. The sensitivity and specificity of the test were found to be 100% (CI95 65.5-100%) and 95% (CI95 81.8-99.1%), respectively, by comparison with broth microdilution as the reference method. The Rapid Polymyxin/Pseudomonas NP test is easy to perform, specific and sensitive, allowing rapid visual observation of results without the requirement any special reading equipment, and results are obtained in 3 h.
Keywords
Anti-Bacterial Agents/pharmacology, Anti-Bacterial Agents/therapeutic use, Drug Resistance, Bacterial, Humans, Microbial Sensitivity Tests, Polymyxins/pharmacology, Polymyxins/therapeutic use, Pseudomonas Infections/drug therapy, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/drug effects, Sensitivity and Specificity, Colistin, Pseudomonas aeruginosa, Rapid diagnostic test, Susceptibility testing
Pubmed
Web of science
Create date
25/04/2020 18:30
Last modification date
09/04/2024 7:14
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