Analysis of glial acidic fibrillary protein in the human entorhinal cortex during aging and in Alzheimer's disease.

Details

Serval ID
serval:BIB_9C87C62E8BFD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of glial acidic fibrillary protein in the human entorhinal cortex during aging and in Alzheimer's disease.
Journal
Proteomics
Author(s)
Porchet R., Probst A., Bouras C., Dráberová E., Dráber P., Riederer B.M.
ISSN
1615-9853 (Print)
ISSN-L
1615-9853
Publication state
Published
Issued date
2003
Volume
3
Number
8
Pages
1476-1485
Language
english
Abstract
Glial fibrillary acidic protein, GFAP, is a major intermediate filament protein of glial cells and major cytoskeletal structure in astrocytes. The entorhinal cortex has a key role in memory function and is one of the first brain areas to reveal hallmark structures of Alzheimer's disease and therefore provides an ideal tissue to investigate incipient neurodegenerative changes. Here we have analyzed age- and disease-related occurrence and composition of GFAP in the human entorhinal cortex by using one- and two-dimensional electrophoresis, Western blots and immunocytochemistry combined with confocal microscopy. A novel monoclonal antibody, GF-02, was characterized that mainly reacted with intact GFAP molecules and indicated that more acidic and soluble GFAP forms were also more susceptible to degradation. GFAP and vimentin increased with aging and in Alzheimer's disease (AD). Two-dimensional electrophoresis and Western blots revealed a complex GFAP pattern, both in aging and AD with different modification and degradation forms. Immunohistochemistry indicated that reactive astrocytes mainly accumulated in relation to neurofibrillary tangles and senile plaques in deeper entorhinal cortex layers. GFAP may be used as an additional but not exclusive diagnostic tool in the evaluation of neurodegenerative diseases because its levels change with age and respond to senile plaque and tangle formation.
Keywords
Aged, Aged, 80 and over, Aging/metabolism, Alzheimer Disease/metabolism, Blotting, Western, Electrophoresis, Gel, Two-Dimensional, Entorhinal Cortex/metabolism, Female, Glial Fibrillary Acidic Protein/metabolism, Humans, Immunohistochemistry, Male, Middle Aged, Precipitin Tests
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 15:03
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