A role for lymphotoxin beta receptor in host defense against Mycobacterium bovis BCG infection.

Details

Serval ID
serval:BIB_9C6B4069A24A
Type
Article: article from journal or magazin.
Collection
Publications
Title
A role for lymphotoxin beta receptor in host defense against Mycobacterium bovis BCG infection.
Journal
European Journal of Immunology
Author(s)
Lucas R., Tacchini-Cottier F., Guler R., Vesin D., Jemelin S., Olleros M.L., Marchal G., Browning J.L., Vassalli P., Garcia I.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
1999
Volume
29
Number
12
Pages
4002-4010
Language
english
Abstract
To investigate the role of membrane lymphotoxin (LT)alpha1 / beta2 and its LTbeta receptor (LTbetaR) in the protective immune response to Mycobacterium bovis bacillus Calmette-Guérin (BCG) infection, we have used a soluble fusion molecule (LTbetaR-IgG1). LTbetaR-Ig treatment interferes with granuloma formation mainly in the spleen by inhibiting macrophage activation and nitric oxide synthase activity. In addition, a large accumulation of eosinophils was observed in the spleen of LTbetaR-Ig-treated infected mice. Decreased blood levels of IFN-gamma and increased IL-4 were also observed, suggesting that the LTbetaR pathway is important in BCG infection to favor a Th1 type of immune response. The treatment of transgenic mice expressing high blood levels of a soluble TNFR1-IgG3 fusion protein with LTbetaR-Ig resulted in a still higher sensitivity to BCG infection, and extensive necrosis in the spleen. In conclusion, these results suggest that the LTbetaR and the TNFR pathways are not redundant in the course of BCG infection and protective granuloma formation: the LTbetaR pathway appears to be important in spleen granuloma formation, whereas the TNFR pathway has a predominant role in other tissues.
Keywords
Animals, Gene Expression Regulation/immunology, Immunity/genetics, Lymphotoxin beta Receptor, Lymphotoxin-alpha/immunology, Lymphotoxin-beta, Membrane Proteins/immunology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Mycobacterium bovis/immunology, Receptors, Tumor Necrosis Factor/genetics, Receptors, Tumor Necrosis Factor/immunology, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/immunology, Transfection, Tuberculosis/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:08
Last modification date
20/08/2019 15:03
Usage data