Protective anti-V antibodies inhibit Pseudomonas and Yersinia translocon assembly within host membranes.

Details

Serval ID
serval:BIB_9C52D5CBA3ED
Type
Article: article from journal or magazin.
Collection
Publications
Title
Protective anti-V antibodies inhibit Pseudomonas and Yersinia translocon assembly within host membranes.
Journal
Journal of Infectious Diseases
Author(s)
Goure J., Broz P., Attree O., Cornelis G.R., Attree I.
ISSN
0022-1899 (Print)
ISSN-L
0022-1899
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
192
Number
2
Pages
218-225
Language
english
Abstract
Pathogenic Yersinia species and Pseudomonas aeruginosa share a similar type III secretion/translocation system. The translocation system consists of 3 secreted proteins, YopB/PopB, YopD/PopD, and LcrV/PcrV; the latter is known to be a protective antigen. In an in vitro assay, the translocation system causes the lysis of erythrocytes infected with wild-type (wt) P. aeruginosa. wt Y. enterocolitica is not hemolytic, but a multiknockout mutant deprived of all the effectors and of YopN ( Delta HOPEMN) is hemolytic. In the presence of antibodies against PcrV and Y. pestis LcrV, the hemolytic activity of P. aeruginosa was inhibited. Similarly, the hemolytic activity of Delta HOPEMN was inhibited in the presence of anti-LcrV antibodies. The assembly of the translocon, composed of PopB/D and YopB/D proteins, was disturbed in immunoprotected erythrocyte membranes, mimicking the phenotypes of V knockout mutants. Thus, protective antibodies against the V antigens of Yersinia species and P. aeruginosa act at the level of the formation of the translocon pore in membranes of infected host cells by blocking the function of LcrV/PcrV. The hemolysis assay could be adapted for high-throughput screening of anti-infectious compounds that specifically target the type III translocon.

Keywords
Cell Membrane/microbiology, DNA Primers, Erythrocyte Membrane/microbiology, Erythrocytes/microbiology, Humans, Polymerase Chain Reaction, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/pathogenicity, Translocation, Genetic, Yersinia/genetics, Yersinia/pathogenicity
Pubmed
Web of science
Create date
25/10/2017 11:05
Last modification date
20/08/2019 16:03
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