Sequence analysis and expression of the attachment and fusion proteins of canine distemper virus wild-type strain A75/17.

Details

Serval ID
serval:BIB_9C3E4D32D22C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sequence analysis and expression of the attachment and fusion proteins of canine distemper virus wild-type strain A75/17.
Journal
Journal of Virology
Author(s)
Cherpillod P., Beck K., Zurbriggen A., Wittek R.
ISSN
0022-538X[print], 0022-538X[linking]
Publication state
Published
Issued date
03/1999
Volume
73
Number
3
Pages
2263-2269
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The biological properties of wild-type A75/17 and cell culture-adapted Onderstepoort canine distemper virus differ markedly. To learn more about the molecular basis for these differences, we have isolated and sequenced the protein-coding regions of the attachment and fusion proteins of wild-type canine distemper virus strain A75/17. In the attachment protein, a total of 57 amino acid differences were observed between the Onderstepoort strain and strain A75/17, and these were distributed evenly over the entire protein. Interestingly, the attachment protein of strain A75/17 contained an extension of three amino acids at the C terminus. Expression studies showed that the attachment protein of strain A75/17 had a higher apparent molecular mass than the attachment protein of the Onderstepoort strain, in both the presence and absence of tunicamycin. In the fusion protein, 60 amino acid differences were observed between the two strains, of which 44 were clustered in the much smaller F2 portion of the molecule. Significantly, the AUG that has been proposed as a translation initiation codon in the Onderstepoort strain is an AUA codon in strain A75/17. Detailed mutation analyses showed that both the first and second AUGs of strain A75/17 are the major translation initiation sites of the fusion protein. Similar analyses demonstrated that, also in the Onderstepoort strain, the first two AUGs are the translation initiation codons which contribute most to the generation of precursor molecules yielding the mature form of the fusion protein.
Keywords
Amino Acid Sequence, Animals, Cells, Cultured, Cercopithecus aethiops, Codon, Distemper Virus, Canine/chemistry, Molecular Sequence Data, Receptors, Virus/chemistry, Receptors, Virus/genetics, Viral Fusion Proteins/chemistry, Viral Fusion Proteins/genetics
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 15:03
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