Human MAF1 targets and represses active RNA polymerase III genes by preventing recruitment rather than inducing long-term transcriptional arrest.

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State: Public
Version: Final published version
Serval ID
serval:BIB_9C3459E28377
Type
Article: article from journal or magazin.
Collection
Publications
Title
Human MAF1 targets and represses active RNA polymerase III genes by preventing recruitment rather than inducing long-term transcriptional arrest.
Journal
Genome research
Author(s)
Orioli A., Praz V., Lhôte P., Hernandez N.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
03/2016
Peer-reviewed
Oui
Volume
26
Number
5
Pages
624-635
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
RNA polymerase III (Pol III) is tightly controlled in response to environmental cues, yet a genomic-scale picture of Pol III regulation and the role played by its repressor MAF1 is lacking. Here, we describe genome-wide studies in human fibroblasts that reveal a dynamic and gene-specific adaptation of Pol III recruitment to extracellular signals in an mTORC1-dependent manner. Repression of Pol III recruitment and transcription are tightly linked to MAF1, which selectively localizes at Pol III loci, even under serum-replete conditions, and increasingly targets transcribing Pol III in response to serum starvation. Combining Pol III binding profiles with EU-labeling and high-throughput sequencing of newly synthesized small RNAs, we show that Pol III occupancy closely reflects ongoing transcription. Our results exclude the long-term, unproductive arrest of Pol III on the DNA as a major regulatory mechanism and identify previously uncharacterized, differential coordination in Pol III binding and transcription under different growth conditions.

Keywords
Cell Line, Humans, Multiprotein Complexes/genetics, Multiprotein Complexes/metabolism, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, Repressor Proteins/genetics, Repressor Proteins/metabolism, TOR Serine-Threonine Kinases/genetics, TOR Serine-Threonine Kinases/metabolism, Transcription, Genetic/physiology
Pubmed
Open Access
Yes
Create date
15/03/2016 20:46
Last modification date
20/08/2019 16:02
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