Expression of B220 on activated T cell blasts precedes apoptosis.

Details

Serval ID
serval:BIB_9C20435DD151
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of B220 on activated T cell blasts precedes apoptosis.
Journal
European journal of immunology
Author(s)
Renno T., Attinger A., Rimoldi D., Hahne M., Tschopp J., MacDonald H.R.
ISSN
0014-2980
Publication state
Published
Issued date
1998
Peer-reviewed
Oui
Volume
28
Number
2
Pages
540-547
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Superantigens are bacterial or viral products that polyclonally activate T cells bearing certain TCR beta chain variable elements. For instance, Vbeta8+ T cells proliferate in response to staphylococcal enterotoxin B (SEB) in vivo and then undergo Fas- and/or TNF-mediated apoptosis. We have recently shown that apoptotic SEB-reactive T cells express the B cell marker B220. Here we report the identification of a novel subset of CD4+ B220+ T cell blasts that are the precursors of these apoptotic cells in SEB-immunized mice. Moreover, we show that the CD4- CD8- B220+ T cells that accumulate in the lymphoid organs of Fas ligand-defective gld mice stably express a form of the B220 molecule which exhibits biochemical similarities to that expressed by activated wild-type T cells, but is distinct from that displayed on the surface of B cells. Surprisingly, we also find a population of CD4+ B220+ pre-apoptotic T cells in FasL-defective gld mice, arguing that these cells can be generated in a Fas-independent fashion. Collectively, our data support a general model whereby upon activation, T cells up-regulate B220 before undergoing apoptosis. When the apoptotic mechanisms are defective, T cells presumably down-regulate their coreceptor molecules but retain expression of B220 as they accumulate in lymphoid organs.
Keywords
Animals, Annexin A5/analysis, Antigens, CD4/analysis, Antigens, CD45/analysis, Antigens, CD45/biosynthesis, Apoptosis/immunology, B-Lymphocytes/immunology, Blotting, Western, Enterotoxins/pharmacology, Lymphocyte Activation, Lymphoproliferative Disorders/genetics, Lymphoproliferative Disorders/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Staphylococcus aureus/immunology, T-Lymphocyte Subsets/enzymology, T-Lymphocyte Subsets/immunology, Up-Regulation/immunology
Pubmed
Web of science
Create date
24/01/2008 16:18
Last modification date
20/08/2019 16:02
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