Synthetic long peptide-based vaccine formulations for induction of cell mediated immunity: A comparative study of cationic liposomes and PLGA nanoparticles.

Details

Serval ID
serval:BIB_9B8CC9ECA180
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Synthetic long peptide-based vaccine formulations for induction of cell mediated immunity: A comparative study of cationic liposomes and PLGA nanoparticles.
Journal
Journal of Controlled Release
Author(s)
Varypataki E.M., Silva A.L., Barnier-Quer C., Collin N., Ossendorp F., Jiskoot W.
ISSN
1873-4995 (Electronic)
ISSN-L
0168-3659
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
226
Pages
98-106
Language
english
Abstract
Nanoparticulate formulations for synthetic long peptide (SLP)-cancer vaccines as alternative to clinically used Montanide ISA 51- and squalene-based emulsions are investigated in this study. SLPs were loaded into TLR ligand-adjuvanted cationic liposomes and PLGA nanoparticles (NPs) to potentially induce cell-mediated immune responses. The liposomal and PLGA NP formulations were successfully loaded with up to four different compounds and were able to enhance antigen uptake by dendritic cells (DCs) and subsequent activation of T cells in vitro. Subcutaneous vaccination of mice with the different formulations showed that the SLP-loaded cationic liposomes were the most efficient for the induction of functional antigen-T cells in vivo, followed by PLGA NPs which were as potent as or even more than the Montanide and squalene emulsions. Moreover, after transfer of antigen-specific target cells in immunized mice, liposomes induced the highest in vivo killing capacity. These findings, considering also the inadequate safety profile of the currently clinically used adjuvant Montanide ISA-51, make these two particulate, biodegradable delivery systems promising candidates as delivery platforms for SLP-based immunotherapy of cancer.
Keywords
Cellular immune response, Synthetic long peptides, TLR ligands, Cationic liposomes, PLGA nanoparticles
Pubmed
Web of science
Create date
20/02/2016 16:53
Last modification date
20/08/2019 15:02
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