Spatial, temporal and subcellular localization of islet-brain 1 (IB1), a homologue of JIP-1, in mouse brain.

Details

Serval ID
serval:BIB_9B6454A8FA23
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatial, temporal and subcellular localization of islet-brain 1 (IB1), a homologue of JIP-1, in mouse brain.
Journal
European Journal of Neuroscience
Author(s)
Pellet J.B., Haefliger J.A., Staple J.K., Widmann C., Welker E., Hirling H., Bonny C., Nicod P., Catsicas S., Waeber G., Riederer B.M.
ISSN
0953-816X
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
12
Number
2
Pages
621-632
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Islet-brain 1 (IB1) was recently identified as a DNA-binding protein of the GLUT2 gene promoter. The mouse IB1 is the rat and human homologue of the Jun-interacting protein 1 (JIP-1) which has been recognized as a key player in the regulation of c-Jun amino-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) pathways. JIP-1 is involved in the control of apoptosis and may play a role in brain development and aging. Here, IB1 was studied in adult and developing mouse brain tissue by in situ hybridization, Northern and Western blot analysis at cellular and subcellular levels, as well as by immunocytochemistry in brain sections and cell cultures. IB1 expression was localized in the synaptic regions of the olfactory bulb, retina, cerebral and cerebellar cortex and hippocampus in the adult mouse brain. IB1 was also detected in a restricted number of axons, as in the mossy fibres from dentate gyrus in the hippocampus, and was found in soma, dendrites and axons of cerebellar Purkinje cells. After birth, IB1 expression peaks at postnatal day 15. IB1 was located in axonal and dendritic growth cones in primary telencephalon cells. By biochemical and subcellular fractionation of neuronal cells, IB1 was detected both in the cytosolic and membrane fractions. Taken together with previous data, the restricted neuronal expression of IB1 in developing and adult brain and its prominent localization in synapses suggest that the protein may be critical for cell signalling in developing and mature nerve terminals.
Keywords
Adaptor Proteins, Signal Transducing, Animals, Brain, Brain Chemistry, Carrier Proteins, Cells, Cultured, Cerebral Cortex, Eye Proteins, Fetal Proteins, Gene Expression Regulation, Developmental, In Situ Hybridization, JNK Mitogen-Activated Protein Kinases, MAP Kinase Signaling System, Mice, Mitogen-Activated Protein Kinases, Nerve Tissue Proteins, Nuclear Proteins, Organ Specificity, Protein Isoforms, Rats, Rats, Wistar, Retina, Reverse Transcriptase Polymerase Chain Reaction, Species Specificity, Subcellular Fractions, Telencephalon, Trans-Activators
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 15:02
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